5' long terminal repeat (LTR)-selective methylation of latently infected HIV-1 provirus that is demethylated by reactivation signals

被引：72

作者：

Ishida, Takaomi ^{[1
]}

Hamano, Akiko ^{[1
]}

Koiwa, Tsukasa ^{[1
]}

Watanabe, Toshiki ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci, Tumor Cell Biol Lab,Minato Ku, Tokyo 1088639, Japan

来源：

RETROVIROLOGY | 2006年 / 3卷 / 1期

关键词：

D O I：

10.1186/1742-4690-3-69

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We previously described selective hypermethylation of the 5'-long terminal repeat (LTR) of HTLV-1 provirus in vivo and in vitro. This prompted us to analyze CpG methylation of the two LTRs of the HIV provirus in chronically infected cell lines. The results demonstrate selective hypermethylation of the 5' LTR of the HIV provirus in ACH-2 cells. Moreover, induction of viral gene expression by TNF-alpha resulted in demethylation of the 5'- LTR. These results suggest that selective epigenetic modification of the 5' LTR of the HIV-1 provirus may be an important mechanism by which proviral activity is suppressed.

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页数：7

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