Molecular basis of autotrophic vs mixotrophic growth in Chlorella sorokiniana

被引：107

作者：

Cecchin, M. ^{[1
]}

Benfatto, S. ^{[1
]}

Griggio, F. ^{[1
]}

Mori, A. ^{[1
]}

Cazzaniga, S. ^{[1
]}

Vitulo, N. ^{[1
]}

Delledonne, M. ^{[1
]}

Ballottari, M. ^{[1
]}

机构：

[1] Univ Verona, Dipartimento Biotecnol, Str Le Grazie 15, I-37134 Verona, Italy

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

DIFFERENTIAL EXPRESSION ANALYSIS; CYCLIC ELECTRON FLOW; CHLAMYDOMONAS-REINHARDTII; CARBON METABOLISM; BINDING PROTEIN; ISOCITRATE LYASE; PHOTOSYNTHESIS; TRANSCRIPTION; ACETATE; LIGHT;

D O I：

10.1038/s41598-018-24979-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

In this work, we investigated the molecular basis of autotrophic vs. mixotrophic growth of Chlorella sorokiniana, one of the most productive microalgae species with high potential to produce biofuels, food and high value compounds. To increase biomass accumulation, photosynthetic microalgae are commonly cultivated in mixotrophic conditions, adding reduced carbon sources to the growth media. In the case of C. sorokiniana, the presence of acetate enhanced biomass, proteins, lipids and starch productivity when compared to autotrophic conditions. Despite decreased chlorophyll content, photosynthetic properties were essentially unaffected while differential gene expression profile revealed transcriptional regulation of several genes mainly involved in control of carbon flux. Interestingly, acetate assimilation caused upregulation of phosphoenolpyruvate carboxylase enzyme, enabling potential recovery of carbon atoms lost by acetate oxidation. The obtained results allowed to associate the increased productivity observed in mixotrophy in C. sorokiniana with a different gene regulation leading to a fine regulation of cell metabolism.

引用

页数：13

共 56 条

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Light Remodels Lipid Biosynthesis in Nannochloropsis gaditana by Modulating Carbon Partitioning between Organelles [J].