Removal of AMPA receptors (AMPARs) from synapses is preceded by transient endocytosis of extrasynaptic AMPARs

被引：178

作者：

Ashby, MC

De La Rue, SA

Ralph, GS

Uney, J

Collingridge, GL

Henley, JM ^{[1
]}

机构：

[1] Univ Bristol, Sch Med Sci, Dept Anat, MRC Ctr Synapt Plast, Bristol BS8 1TD, Avon, England

[2] Univ Bristol, Div Med, MRC Ctr Synapt Plast, Bristol BS2 8HW, Avon, England

来源：

JOURNAL OF NEUROSCIENCE | 2004年 / 24卷 / 22期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

AMPARs; trafficking; imaging; cell surface; pHluorin; hippocampus;

D O I：

10.1523/JNEUROSCI.1042-04.2004

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

AMPA receptors (AMPARs) are dynamically regulated at synapses, but the time course and location of their exocytosis and endocytosis are not known. Therefore, we have used ecliptic pHluorin-tagged glutamate receptor 2 to visualize changes in AMPAR surface expression in real time. We show that synaptic and extrasynaptic AMPARs respond very differently to NMDA receptor activation; there is a rapid internalization of extrasynaptic AMPARs that precedes the delayed removal of synaptic AMPARs.

引用

页码：5172 / 5176

页数：5

共 22 条

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Banker G., 1998, Culturing nerve cells

[2]

Regulation of AMPA receptor endocytosis by a signaling mechanism shared with LTD [J].