Determination of melengestrol acetate in supercritical fluid-solid phase extracts of bovine fat tissue by HPLC-UV and CC-MS

被引：10

作者：

Parks, OW ^{[1
]}

Shadwell, RJ ^{[1
]}

Lightfield, AR ^{[1
]}

Maxwell, RJ ^{[1
]}

机构：

[1] USDA ARS,EASTERN REG RES CTR,WYNDMOOR,PA 19038

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1996年 / 34卷 / 08期

关键词：

D O I：

10.1093/chromsci/34.8.353

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is developed for the determination of melengestrol acetate in bovine fat tissue at or less than the established tolerance level of 25 ppb. The procedure uses a combination of supercritical fluid extraction (SFE) and solid-phase extraction (SPE) techniques to produce an extract suitable for analysis with either high-performance liquid chromatography with ultraviolet detection or gas chromatography-mass spectrometry. Overall recovery of the analyte from bovine fat tissue is 99.4% with a coefficient of variation of 4.14%. The SFE-SPE procedure uses a total of 12 mL of organic solvent per fat tissue sample versus more than 1.7 L consumed in current extraction procedures.

引用

页码：353 / 357

页数：5

共 9 条

[1]

*ASS OFF AN CHEM, 1990, OFF METH AN, P629

[2] DETERMINATION OF MELENGESTROL ACETATE IN BOVINE-TISSUES BY AUTOMATED COUPLED-COLUMN NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

CHICHILA, TMP ;

EDLUND, PO ;

HENION, JD ;

WILSON, R ;

EPSTEIN, RL .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 488 (02) :389-406

[3]

MAGARD MA, 1995, J AGR FOOD CHEM, V43, P114, DOI 10.1021/jf00049a022

[4]

PARKS OW, 1995, J CHROMATOGR SCI, V33, P654

[5] ISOLATION OF SULFONAMIDES FROM FORTIFIED CHICKEN TISSUES WITH SUPERCRITICAL CO2 AND IN-LINE ADSORPTION [J].

PARKS, OW ;

MAXWELL, RJ .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1994, 32 (07) :290-293

[6]

STOLKER AAM, 1994, P 5 INT S SUP FLUID

[7]

*USDA, 1991, AN CHEM LAB GUID RES

[8]

1991, FED REGISTER, V56, P7849

[9]

1994, FOOD CHEM NEWS 0815, P34

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