Donor-donor energy-migration measurements of dimeric DsbC labeled at its N-terminal amines with fluorescent probes: A study of protein unfolding

被引：25

作者：

Duan, XJ

Zhao, Z

Ye, JP

Ma, HM ^{[1
]}

Xia, AD

Yang, GQ

Wang, CC

机构：

[1] Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China

[2] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 32期

关键词：

amines; analytical methods; energy transfer; fluorescent probes; protein folding;

D O I：

10.1002/anie.200460072

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The folding/unfolding of a protein structure has been studied through measuring the donor-donor energy migration between two fluorescent probes (F) coupled selectively to the N termini of the homodimeric DsbC protein (see picture). The fluorometric strategy provides a convenient and reliable method to investigate conformational changes in dimeric proteins.

引用

收藏

页码：4216 / 4219

页数：4

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