Lactose hydrolysis by β-galactosidase enzyme: optimization using response surface methodology

被引：54

作者：

Das, Bipasha ^{[1
]}

Roy, Ananda Prasad ^{[1
]}

Bhattacharjee, Sangita ^{[2
]}

Chakraborty, Sudip ^{[3
]}

Bhattacharjee, Chiranjib ^{[1
]}

机构：

[1] Jadavpur Univ, Dept Chem Engn, Kolkata 700032, W Bengal, India

[2] Heritage Inst Technol, Dept Chem Engn, Kolkata 700107, W Bengal, India

[3] Univ Calabria, Dept Chem Engn & Mat, I-87036 Arcavacata Di Rende, CS, Italy

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2015年 / 121卷

关键词：

Lactose hydrolysis; beta-galactosidase; Immobilized enzyme; Calcium alginate; Response surface methodology; IMMOBILIZATION; STABILITY; WHEY;

D O I：

10.1016/j.ecoenv.2015.03.024

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

In the present study, it was aimed to optimize the process of lactose hydrolysis using free and immobilized p-galactosidase to produce glucose and galactose. Response surface methodology (RSM) by central composite design (CCD) was employed to optimize the degree of hydrolysis by varying three parameters, temperature (15-45 degrees C), solution pH (5-9) and beta-galactosidase enzyme concentration (2-8 mg/mL) for free mode of analysis and sodium alginate concentration (2-4%), calcium chloride concentration (3-6%) and enzyme concentration (2-8 mg/mL) for immobilized process. Based on plots and variance analysis, the optimum operational conditions for maximizing lactose hydrolysis were found to be temperature (35.5 degrees C), pH (6.7) and enzyme concentration (6.7 mg/mL) in free mode and sodium alginate concentration (3%), calcium chloride concentration (5.9%) and enzyme concentration (5.2 mg/mL) in immobilized mode. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：244 / 252

页数：9

共 25 条

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