Monitoring cellular state transitions in a production-scale CHO-cell process using an electronic nose

被引：29

作者：

Bachinger, T

Riese, U

Eriksson, R

Mandenius, CF ^{[1
]}

机构：

[1] Linkoping Univ, Swedish Sensor Ctr, Dept Phys & Measurement Technol, S-58183 Linkoping, Sweden

[2] Linkoping Univ, Appl Phys Lab, S-58183 Linkoping, Sweden

[3] Pharmacia & Upjohn Inc, Cell Culture Prod, S-11287 Stockholm, Sweden

来源：

JOURNAL OF BIOTECHNOLOGY | 2000年 / 76卷 / 01期

关键词：

electronic nose; CHO-cell culture; recombinant factor VIII; bioreactor monitoring;

D O I：

10.1016/S0168-1656(99)00179-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An electronic nose is used to monitor the bioreactor off-gas composition in perfused cultivations of a CHO-cell line producing recombinant human blood coagulation factor VIII. The applicability of the electronic nose for monitoring cellular state transitions and process control is explained. Tt is shown that the instrument can reveal characteristic process states related to product and lactate formation, and detect microbial infections in a very early stage of the infection. The visualization of ideal process conditions is realized by using principal component analysis (PCA) and the on-line applicability of this method is outlined. The results illustrate the potential of the electronic nose as on-line sensor for ensuring product and process quality in production-scale bioprocesses. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：61 / 71

页数：11

共 14 条

[1]

Bachinger T., 1998, Seminars in Food Analysis, V3, P85

[2] Estimation of biomass and specific growth rate in a recombinant Escherichia coli batch cultivation process using a chemical multisensor array [J].