Lonicera hypoglauca Inhibits Xanthine Oxidase and Reduces Serum Uric Acid in Mice

被引:39
作者
Chien, Shih-Chang [2 ]
Yang, Chen-Wei [1 ]
Tseng, Yen-Hsueh [1 ]
Tsay, Hsin-Sheng [3 ]
Kuo, Yueh-Hsiung [2 ,4 ,5 ,6 ,7 ]
Wang, Sheng-Yang [1 ]
机构
[1] Natl Chung Hsing Univ, Dept Forestry, Taichung 402, Taiwan
[2] China Med Univ, Sch Chinese Med Resources, Taichung, Taiwan
[3] Chaoyang Univ Technol, Grad Inst Biotechnol, Taichung, Taiwan
[4] Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115, Taiwan
[5] China Med Univ, Coll Pharm, Tsuzuki Inst Tradit Med, Taichung, Taiwan
[6] Natl Taiwan Univ, Dept Chem, Taipei 10764, Taiwan
[7] Natl Taiwan Univ, Ctr Food & Biomol, Taipei 10764, Taiwan
关键词
Lonicera hypoglauca; Caprifoliaceae; hyperuricemia; xanthine oxidase inhibitor; loniceraflavone; OXONATE-INDUCED MICE; JAPONICA; LEAVES; RATS; OIL;
D O I
10.1055/s-0029-1185300
中图分类号
Q94 [植物学];
学科分类号
071001 [植物学];
摘要
Xanthine oxidase (XOD) catalyzes the oxidation of hypoxanthine to xanthine and then to uric acid, and is a key enzyme in the pathogenesis of hyperuricemia. The ability of extracts of Lonicera hypoglauca (Caprifoliaceae) to inhibit XOD was investigated in this study. An ethanol extract (LH-crude) of the leaves of L. hypoglauca and its derived EtOAc soluble sub-fractions (LH-EA) significantly inhibited XOD activity, with IC50 values for LH-crude and LH-EA of 48.8 and 35.2 mu g/mL. Moreover, LH-EA reduced serum urate levels in vivo in a potassium oxonate-induced hyperuricemic mouse model, by 70.1% and 93.7% of the hyperuricemic untreated group at doses of 300 and 500 mg/kg of LH-EA, respectively. Finally, we used bioactivity-guided fractionation to isolate a new bisflavonoid, loniceraflavone, which showed significant inhibition of XOD (IC50 = 0.85 mu g/mL). These results suggest that L. hypoglauca and its extracts may have a considerable potential for development as an anti-hyperuricemia agent for clinical application.
引用
收藏
页码:302 / 306
页数:5
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