Involvement of ppGpp, ribosome modulation factor, and stationary phase-specific sigma factor σS in the decrease in cell viability caused by spermidine

被引：13

作者：

Apirakaramwong, A

Kashiwagi, K

Raj, VS

Sakata, K

Kakinuma, Y

Ishihama, A

Igarashi, K

机构：

[1] Chiba Univ, Fac Pharmaceut Sci, Inage Ku, Chiba 2638522, Japan

[2] Natl Inst Genet, Dept Mol Genet, Shizuoka 4110801, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 264卷 / 03期

关键词：

D O I：

10.1006/bbrc.1999.1556

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Accumulation of spermidine in Escherichia coli causes a decrease in cell viability at the late stationary phase of cell growth. The mechanism underlying this effect has been studied. Spermidine accumulation caused an increase in the level of ppGpp and a decrease in ribosome modulation factor (RMF) and stationary phase-specific sigma factor sigma(S), both of which are believed to be involved in cell viability. Transformation of E. coli with the gene for stringent factor, which synthesizes ppGpp, also caused a significant decrease in the levels of RMF and sigma(S) factor and a decrease in cell viability. The results strongly suggest that the accumulation of ppGpp is also involved in the decrease in cell viability and that the sigma(S) factor assists the function of RMF in cell viability. (C) 1999 Academic Press.

引用

页码：643 / 647

页数：5

共 26 条

[1] Enhancement of cell death due to decrease in Mg2+ uptake by OmpC (cation-selective porin) deficiency in ribosome modulation factor-deficient mutant [J].