Pseudobasophilia as an erroneous white blood cell differential count with a discrepancy between automated cell counters: report of two cases

被引:19
作者
Hur, M
Lee, YK
Lee, KM
Kim, HJ
Cho, HI
机构
[1] Seoul Natl Univ, Coll Med, Dept Lab Med, Seoul 110744, South Korea
[2] Hallym Univ, Coll Med, Dept Lab Med, Seoul, South Korea
[3] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Lab Med, Seoul, South Korea
来源
CLINICAL AND LABORATORY HAEMATOLOGY | 2004年 / 26卷 / 04期
关键词
automated counter; differential count; discrepancy; pseudobasophilia;
D O I
10.1111/j.1365-2257.2004.00615.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We report two cases that showed erroneous white blood cell differential counts by automated cell counters. Each case showed an interesting discrepancy of differential count between cell counters, and marked pseudobasophilia was observed by one of the two counters. The first patient was a 44-year-old female who suffered from multiple myeloma for more than one and a half years. Increased myeloma cells (43%) in peripheral blood were counted as basophils by the ADVIA 120, and as monocytes by SE-9000, respectively. The second patient was a 72-year-old female diagnosed as having chronic myelomonocytic leukemia. Dysgranulopoietic neutrophils (50%) and monocytes (31%) were increased in the peripheral blood. Dysgranulopoietic neutrophils were counted as basophils by STKS. In contrast, about half of the increased monocytes were counted as neutrophils by the ADVIA 120. These interesting findings highlight the importance of microscopic examination of the blood film in routine laboratory practice, and automated cell counters, especially for the hematologic patients, cannot completely substitute for it. These results also imply that at least some subpopulations with different membrane or cytoplasmic properties may exist even in the similarly classified cells.
引用
收藏
页码:287 / 290
页数:4
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