Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions

被引：218

作者：

Przybylo, Magdalena ^{[1
]}

Sykora, Jan ^{[1
]}

Humpolickova, Jana ^{[1
]}

Benda, Ales ^{[1
]}

Zan, Anna ^{[1
]}

Hof, Martin ^{[1
]}

机构：

[1] Acad Sci Czech Republ, J Heyrovsky Inst Phys Chem, Prague 18223 8, Czech Republic

来源：

LANGMUIR | 2006年 / 22卷 / 22期

关键词：

D O I：

10.1021/la061934p

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The lateral diffusion coefficients of a BODIPY tail-labeled lipid in two model systems, namely, free-standing giant unilamellar vesicles (GUVs) and supported phospholipid bilayers (SPBs), were determined by fluorescence correlation spectroscopy (FCS) using the Z-scan approach. For the first time, the performed measurements on 1,2-dioleoyl-sn-glycero-3-phosphocholine ( DOPC) bilayers maintain exactly the same experimental conditions for both systems, which allows for a quantitative comparison of lipid diffusion in these two commonly used model membranes. The results obtained revealed that the lipid mobility in free-standing bilayers (D = 7.8 +/- 0.8 mu m(2) s(-1)) is significantly higher than in the bilayer created on the solid support (mica) (D = 3.1 +/- 0.3 mu m(2) s(-1)).

引用

页码：9096 / 9099

页数：4

共 23 条

[1] LIPOSOME ELECTROFORMATION [J].

ANGELOVA, MI ;

DIMITROV, DS .

FARADAY DISCUSSIONS, 1986, 81 :303-+

[2] Sterol structure determines the separation of phases and the curvature of the liquid-ordered phase in model membranes [J].

Bacia, K ;

Schwille, P ;

Kurzchalia, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (09) :3272-3277

[3] How to determine diffusion coefficients in planar phospholipid systems by confocal fluorescence correlation spectroscopy [J].

Benda, A ;

Benes, M ;

Marecek, V ;

Lhotsky, A ;

Hermens, WT ;

Hof, M .

LANGMUIR, 2003, 19 (10) :4120-4126

[4]

BENDA A, IN PRESS LANGMUIR

[5] Surface-dependent transitions during self-assembly of phospholipid membranes on mica, silica, and glass [J].

Benes, M ;

Billy, D ;

Benda, A ;

Speijer, H ;

Hof, M ;

Hermens, WT .

LANGMUIR, 2004, 20 (23) :10129-10137

[6] Muscovite (mica) allows the characterisation of supported Bilayers by ellipsometry and confocal fluorescence correlation spectroscopy [J].

Benes, M ;

Billy, D ;

Hermens, WT ;

Hof, M .

BIOLOGICAL CHEMISTRY, 2002, 383 (02) :337-341

[7] Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesicles [J].

Doeven, MK ;

Folgering, JHA ;

Krasnikov, V ;

Geertsma, ER ;

van den Bogaart, G ;

Poolman, B .

BIOPHYSICAL JOURNAL, 2005, 88 (02) :1134-1142

[8] Giant liposomes in physiological buffer using electroformation in a flow chamber [J].

Estes, DJ ;

Mayer, M .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2005, 1712 (02) :152-160

[9] Optical saturation in fluorescence correlation spectroscopy under continuous-wave and pulsed excitation [J].

Gregor, I ;

Patra, D ;

Enderlein, J .

CHEMPHYSCHEM, 2005, 6 (01) :164-170

[10] Asymmetric molecular friction in supported phospholipid bilayers revealed by NMR measurements of lipid diffusion [J].

Hetzer, M ;

Heinz, S ;

Grage, S ;

Bayerl, TM .

LANGMUIR, 1998, 14 (05) :982-984

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