Sedum sarmentosum Bunge extract exerts renal anti-fibrotic effects in vivo and in vitro

被引：34

作者：

Bai, Yongheng ^{[1
]}

Lu, Hong ^{[2
]}

Zhang, Ge ^{[3
]}

Wu, Cunzao ^{[4
]}

Lin, Chengcheng ^{[1
]}

Liang, Yong ^{[1
]}

Chen, Bicheng ^{[1
]}

机构：

[1] Wenzhou Med Univ, Affiliated Hosp 1, Wenzhou Key Lab Surg, Wenzhou 325000, Peoples R China

[2] Wenzhou Med Univ, Affiliated Hosp 1, Dept Lab Med, Wenzhou 325000, Peoples R China

[3] Peoples Hosp Luzhou City, Dept Orthoped, Luzhou 646000, Peoples R China

[4] Wenzhou Med Univ, Affiliated Hosp 1, Transplantat Ctr, Wenzhou 325000, Peoples R China

来源：

LIFE SCIENCES | 2014年 / 105卷 / 1-2期

关键词：

Sedum sarmentosum Bunge; Epithelial-to-mesenchymal transition; Matrix accumulation; Transforming growth factor-beta 1; Renal fibrosis; TO-MESENCHYMAL TRANSITION; CHINESE NATURAL MEDICINES; INTERSTITIAL FIBROSIS; URETERAL OBSTRUCTION; MEGASTIGMANE GLYCOSIDES; BIOACTIVE CONSTITUENTS; ABSOLUTE STRUCTURES; CANCER CELLS; APOPTOSIS; GROWTH;

D O I：

10.1016/j.lfs.2014.04.013

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Aims: Sedum sarrnentosum Bunge, a traditional Chinese herbal medicine, has a wide range of clinical effects, including anti-oxidation, anti-inflammation, and anti-cancer properties. In this study, we determined whether S. sarmentosum Bunge Extract (SSBE) has anti-fibrotic effects on renal tissues. Main methods: We investigated the effects of SSBE on aristolochic acid (AA)-induced injury to renal tubular epithelial cells (RTECs) in vitro and unilateral ureteral obstruction (UUO)-induced renal fibrosis in vivo by evaluating epithelial-to-mesenchymal transition (EMT) and the accumulation of extracellular matrix (ECM) components. Furthermore, we examined the expression levels of TGF-beta 1 and its receptor. Key findings: In cultured RTECs (NRK-52E), AA promoted renal EMT and ECM accumulation by up-regulating the expression of mesenchymal markers and ECM components and by down-regulating the expression of epithelial markers. In addition, AA induced an imbalance between MMP-2 and TIMP-2 and enhanced expression of TGF-beta 1 and its receptor. SSBE treatment significantly inhibited AA-induced TGF-beta 1 expression and prevented the induction of EMT and deposition of ECM. In the UUO rats, tubular injury and interstitial fibrosis were obviously increased. SSBE administration protected renal function, as indicated by reduced serum creatinine levels, and alleviated renal interstitial fibrosis. These anti-fibrotic effects were associated with a reduction in TGF-beta 1 expression and inhibition of EMT and ECM accumulation. Significance: These findings suggest that SSBE may have therapeutic potential for fibrotic kidney diseases. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：22 / 30

页数：9

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