Regulation of AE1 anion exchanger and H+-ATPase in rat cortex by acute metabolic acidosis and alkalosis

被引:105
作者
Sabolic, I
Brown, D
Gluck, SL
Alper, SL
机构
[1] BETH ISRAEL HOSP, MOL MED UNIT, BOSTON, MA 02215 USA
[2] BETH ISRAEL HOSP, RENAL UNIT, BOSTON, MA 02215 USA
[3] INST MED RES & OCCUPAT HLTH, ZAGREB, CROATIA
[4] MASSACHUSETTS GEN HOSP, RENAL UNIT, BOSTON, MA 02114 USA
[5] HARVARD UNIV, SCH MED, DEPT PATHOL, BOSTON, MA 02115 USA
[6] HARVARD UNIV, SCH MED, DEPT CELL BIOL, BOSTON, MA 02115 USA
[7] HARVARD UNIV, SCH MED, DEPT MED, BOSTON, MA 02115 USA
[8] WASHINGTON UNIV, SCH MED, DEPT CELL BIOL, ST LOUIS, MO 63110 USA
[9] WASHINGTON UNIV, SCH MED, DEPT MED, ST LOUIS, MO 63110 USA
关键词
RABBIT COLLECTING DUCT; INTERCALATED CELLS; KIDNEY BAND-3; EPITHELIAL-CELLS; DEPLETION; MEMBRANE; IMMUNOLOCALIZATION; COLOCALIZATION; LOCALIZATION; EXPRESSION;
D O I
10.1038/ki.1997.16
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
The cortical collecting duct (CCD) mediates net secretion or reabsorption of protons according to systemic acid/base status. Using indirect immunofluorescence, we examined the localization and abundance of the vacuolar H+-ATPase and the AE1 anion exchanger in intercalated cells (IC) of rat kidney connecting segment (CNT) and CCD during acute (6 hr) metabolic (NH4Cl) acidosis and respiratory (NaHCO3) alkalosis. AE1 immunostaining intensify quantified by confocal microscopy was elevated in metabolic acidosis and substantially reduced in metabolic alkalosis. AE1 immunostaining was restricted to Type A IC in all conditions, and the fraction of AE1(+) IC was unchanged in CNT and CCD. Metabolic acidosis was accompanied by redistribution of H+-ATPase immunostaining towards the apical surface of IC, and metabolic alkalosis was accompained by H+-ATPase redistribution towards the basal surface of IC. Therefore, acute metabolic acidosis produced changes consistent with increased activity of Type A IC and decreased activity of Type B IC, whereas acute metabolic alkalosis produced changes corresponding to increased activity of Type B IC and decreased activity of Type A IC. These data demonstrate that acute systemic acidosis and alkalosis modulate the cellular distribution of two key transporters involved in proton secretion in the distal nephron.
引用
收藏
页码:125 / 137
页数:13
相关论文
共 50 条
[1]  
ALPER SL, 1995, J AM SOC NEPHROL, V6, P372
[2]   FODRIN-ANKYRIN CYTOSKELETON OF CHOROID-PLEXUS PREFERENTIALLY COLOCALIZES WITH APICAL NA+K+-ATPASE RATHER THAN WITH BASOLATERAL ANION-EXCHANGER AE2 [J].
ALPER, SL ;
STUARTTILLEY, A ;
SIMMONS, CF ;
BROWN, D ;
DRENCKHAHN, D .
JOURNAL OF CLINICAL INVESTIGATION, 1994, 93 (04) :1430-1438
[3]   SUBTYPES OF INTERCALATED CELLS IN RAT-KIDNEY COLLECTING DUCT DEFINED BY ANTIBODIES AGAINST ERYTHROID BAND-3 AND RENAL VACUOLAR H+-ATPASE [J].
ALPER, SL ;
NATALE, J ;
GLUCK, S ;
LODISH, HF ;
BROWN, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5429-5433
[4]   THE BAND 3-RELATED AE ANION-EXCHANGER GENE FAMILY [J].
ALPER, SL .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 1994, 4 (5-6) :265-281
[5]  
ALPER SL, 1995, J AM SOC NEPHROL, V6, P371
[6]   EXPRESSION AND DISTRIBUTION OF RENAL VACUOLAR PROTON-TRANSLOCATING ADENOSINE-TRIPHOSPHATASE IN RESPONSE TO CHRONIC ACID AND ALKALI LOADS IN THE RAT [J].
BASTANI, B ;
PURCELL, H ;
HEMKEN, P ;
TRIGG, D ;
GLUCK, S .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (01) :126-136
[7]   ANISOTROPY DECAY MEASUREMENT OF SEGMENTAL DYNAMICS OF THE ANION-BINDING DOMAIN IN ERYTHROCYTE BAND-3 [J].
BICKNESE, S ;
ROSSI, M ;
THEVENIN, B ;
SHOHET, SB ;
VERKMAN, AS .
BIOCHEMISTRY, 1995, 34 (33) :10645-10651
[8]   Depletion of intercalated cells from collecting ducts of carbonic anhydrase II-deficient (CAR2 null) mice [J].
Breton, S ;
Alper, SL ;
Gluck, SL ;
Sly, WS ;
Barker, JE ;
Brown, D .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY, 1995, 269 (06) :F761-F774
[9]   REGIONAL AND SEGMENTAL LOCALIZATION OF AE2 ANION-EXCHANGER MESSENGER-RNA AND PROTEIN IN RAT-KIDNEY [J].
BROSIUS, FC ;
NGUYEN, K ;
STUARTTILLEY, AK ;
HALLER, C ;
BRIGGS, JP ;
ALPER, SL .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY, 1995, 269 (04) :F461-F468
[10]  
BROSIUS FC, 1989, J BIOL CHEM, V264, P7784