Complete nitrification by Nitrospira bacteria

被引：2442

作者：

Daims, Holger ^{[1
]}

Lebedeva, Elena V. ^{[2
]}

Pjevac, Petra ^{[1
]}

Han, Ping ^{[1
]}

Herbold, Craig ^{[1
]}

Albertsen, Mads ^{[3
]}

Jehmlich, Nico ^{[4
]}

Palatinszky, Marton ^{[1
]}

Vierheilig, Julia ^{[1
]}

Bulaev, Alexandr ^{[2
]}

Kirkegaard, Rasmus H. ^{[3
]}

von Bergen, Martin ^{[4
,5
]}

Rattei, Thomas ^{[6
]}

Bendinger, Bernd ^{[7
]}

Nielsen, Per H. ^{[3
]}

Wagner, Michael ^{[1
]}

机构：

[1] Univ Vienna, Div Microbial Ecol, Dept Microbiol & Ecosyst Sci, A-1090 Vienna, Austria

[2] Russian Acad Sci, Biotechnol Res Ctr, Winogradsky Inst Microbiol, Moscow 119071, Russia

[3] Aalborg Univ, Dept Chem & Biosci, Ctr Microbial Communities, DK-9220 Aalborg, Denmark

[4] UFZ Helmholtz Ctr Environm Res, Dept Prote, D-04318 Leipzig, Germany

[5] UFZ Helmholtz Ctr Environm Res, Dept Metabol, D-04318 Leipzig, Germany

[6] Univ Vienna, Div Computat Syst Biol, Dept Microbiol & Ecosyst Sci, A-1090 Vienna, Austria

[7] Hamburg Univ Technol, DVGW Forsch Stelle TUHH, D-21073 Hamburg, Germany

来源：

NATURE | 2015年 / 528卷 / 7583期

基金：

奥地利科学基金会; 欧洲研究理事会;

关键词：

AMMONIA-OXIDIZING BACTERIUM; COMPLETE GENOME SEQUENCE; 16S RIBOSOMAL-RNA; IN-SITU; OXIDATION-KINETICS; HIGH-ACCURACY; SP-NOV; EVOLUTION; SOIL; ARCHAEA;

D O I：

10.1038/nature16461

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Nitrification, the oxidation of ammonia via nitrite to nitrate, has always been considered to be a two-step process catalysed by chemolithoautotrophic microorganisms oxidizing either ammonia or nitrite. No known nitrifier carries out both steps, although complete nitrification should be energetically advantageous. This functional separation has puzzled microbiologists for a century. Here we report on the discovery and cultivation of a completely nitrifying bacterium from the genus Nitrospira, a globally distributed group of nitrite oxidizers. The genome of this chemolithoautotrophic organism encodes the pathways both for ammonia and nitrite oxidation, which are concomitantly expressed during growth by ammonia oxidation to nitrate. Genes affiliated with the phylogenetically distinct ammonia monooxygenase and hydroxylamine dehydrogenase genes of Nitrospira are present in many environments and were retrieved on Nitrospira-contigs in new metagenomes from engineered systems. These findings fundamentally change our picture of nitrification and point to completely nitrifying Nitrospira as key components of nitrogen-cycling microbial communities.

引用

页码：504 / +

页数：20

共 92 条

[1]

Genome sequences of rare, uncultured bacteria obtained by differential coverage binning of multiple metagenomes [J].