Nascent pectin formed in Golgi apparatus of pea epicotyls by addition of uronic acids has different properties from nascent pectin at the stage of galactan elongation

被引：6

作者：

Abdel-Massih, Roula M. ^{[1
]}

Rizkallah, Hind D.

Al-Din, Rabih Saif

Baydoun, Elias A. -H.

Brett, Christopher T.

机构：

[1] Univ Balamand, Fac Sci, Dept Biol, Al Koura, Lebanon

[2] Amer Univ Beirut, Dept Biol, Beirut, Lebanon

[3] Univ Glasgow, Inst Biomed & Life Sci, Plant Mol Sci Grp, Glasgow G12 8QQ, Lanark, Scotland

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2007年 / 164卷 / 01期

关键词：

pectin; Pisum sativum; polygalacturonan; protein; xyloglucan;

D O I：

10.1016/j.jplph.2006.06.013

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Microsomal. membranes were prepared from etiolated pea (Pisum sativum L.) epicotyls and used to form nascent [Uronic acid-C-14] pectin. The enzyme products were characterized by selective enzymic degradation, get permeation chromatography and analysis of cellulose binding properties. The product obtained had a molecular weight of around 40 kDa, which was significantly tower than that of nascent [Gal-C-14] pectin prepared from the same tissues. It is composed mainly of polygalacturonan and perhaps also rhamnogalacturonan (RG-1). Evidence was obtained for the presence of a protein attached to the nascent [Uronic acid-C-14] pectin, but it was unaffected by endoglucanase and did not bind to cellulose. Hence, no xyloglucan appeared to be attached to the nascent [Uronic acid-C-14] pectin. A model. is proposed in which xyloglucan is attached to nascent pectin after formation of homogalacturonan, but before the pectin leaves the Golgi apparatus. (c) 2006 Elsevier GmbH. All rights reserved.

引用

页码：1 / 10

页数：10

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