Crystallization and preliminary X-ray studies of mouse tumor necrosis factor

被引：2

作者：

Baeyens, KJ

DeBondt, HL

Raeymaekers, A

DeRanter, CJ

Fiers, W

机构：

[1] VIB,MOL BIOL LAB,B-9000 GHENT,BELGIUM

[2] STATE UNIV GHENT,B-9000 GHENT,BELGIUM

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1997年 / 53卷

关键词：

D O I：

10.1107/S090744499601534X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Well diffracting crystals of recombinant mouse tumor necrosis factor (mTNF) have been obtained. The sitting-drop vapor-diffusion method was used to grow crystals suitable for X-ray studies from solutions containing methoxypolyethylene glycol 2000 and isopropanol as precipitants. The crystals belong to the space group P1 with unit-cell dimensions a = 49.40, b = 48.24, c = 51.13 Angstrom, alpha = 115.06, beta = 103.32, gamma = 91.27 degrees and one trimer in the asymmetric unit. Crystals are stable to X-rays and diffract beyond 2.0 Angstrom. Cooling the crystal during data collection is necessary since crystals dissolve at room temperature.

引用

页码：329 / 330

页数：2

共 12 条

[1] [Anonymous], BIOL THERAPY CANC
[2] Brunger A. T., 1992, X PLOR VERSION 3 1 S
[3] ECK MJ, 1989, J BIOL CHEM, V264, P17595
[4] TNF alpha is an effective therapeutic target for rheumatoid arthritis
Feldmann, M
Brennan, FM
Elliott, MJ
Williams, RO
Maini, RN
[J]. RECEPTOR ACTIVATION BY ANTIGENS, CYTOKINES, HORMONES, AND GROWTH FACTORS, 1995, 766 : 272 - 278
[5] MOLECULAR-CLONING OF MOUSE-TUMOR NECROSIS FACTOR CDNA AND ITS EUKARYOTIC EXPRESSION
FRANSEN, L
MULLER, R
MARMENOUT, A
TAVERNIER, J
VANDERHEYDEN, J
KAWASHIMA, E
CHOLLET, A
TIZARD, R
VANHEUVERSWYN, H
VANVLIET, A
RUYSSCHAERT, MR
FIERS, W
[J]. NUCLEIC ACIDS RESEARCH, 1985, 13 (12) : 4417 - 4429
[6] SPARSE-MATRIX SAMPLING - A SCREENING METHOD FOR CRYSTALLIZATION OF PROTEINS
JANCARIK, J
KIM, SH
[J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 : 409 - 411
[7] STRUCTURE OF TUMOR NECROSIS FACTOR
JONES, EY
STUART, DI
WALKER, NPC
[J]. NATURE, 1989, 338 (6212) : 225 - 228
[8] Lejeune F, 1995, J Infus Chemother, V5, P73
[9] SOLVENT CONTENT OF PROTEIN CRYSTALS
MATTHEWS, BW
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) : 491 - +
[10] MURATA Y, 1995, J GASTROENTEROL, V8, P56

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