Improves method for the detection of cytokeratin 19-positive cells in the peripheral blood of breast cancer patients

被引：7

作者：

Alvero, AB

Burtness, BA

Ercan, AG

Sapi, E

机构：

[1] Univ New Haven, Dept Biol & Environm Sci, West Haven, CT 06516 USA

[2] Yale Univ, Sch Med, Dept Therapeut Radiol, New Haven, CT 06510 USA

[3] Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06510 USA

[4] Yale Univ, Sch Med, Dept Obstet & Gynecol, New Haven, CT 06510 USA

来源：

LABORATORY INVESTIGATION | 2004年 / 84卷 / 05期

关键词：

breast cancer; micrometastasis; circulating tumor cells; real-time RT-PCR; cytokeratin; 19; peripheral blood;

D O I：

10.1038/labinvest.3700086

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

To determine the correlation of the levels of circulating tumor cells (CTC) in breast cancer patients with clinical parameters like disease-free survival and response to therapy, we employed a previously reported quantitative real-time reverse-transcription polymerase chain reaction (QRT-PCR) assay for cytokeratin-19 (Ck19; Ann Oncol 2001; 12: 39-46). Our preliminary results based on this assay (Protocol A) prompted us to optimize the experimental conditions. Here we report an improved assay (Protocol 13), which more sensitively and specifically detects CTC in breast cancer patients.

引用

页码：658 / 661

页数：4

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