Rapid and Highly Sensitive Neuraminidase Subtyping of Avian Influenza Viruses by Use of a Diagnostic DNA Microarray

被引：30

作者：

Gall, Astrid ^{[1
]}

Hoffmann, Bernd ^{[1
]}

Harder, Timm ^{[1
]}

Grund, Christian ^{[1
]}

Ehricht, Ralf ^{[2
]}

Beer, Martin ^{[1
]}

机构：

[1] Friedrich Loeffler Inst, Inst Diagnost Virol, D-17493 Greifswald, Germany

[2] Clondiag GmbH, D-07749 Jena, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 09期

关键词：

UNIVERSAL PRIMER SET; A VIRUSES; RT-PCR; AMPLIFICATION; GENE;

D O I：

10.1128/JCM.00850-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rapid neuraminidase subtyping of avian influenza viruses from diagnostic samples is crucial considering the existence of permanently emerging and evolving strains. Here we report an easy-to-use, low-cost microarray for neuraminidase subtyping following fragment amplification by a generic, neuraminidase-specific reverse transcription-PCR (RT-PCR). This method enables highly specific characterization with a sensitivity equal to that of matrix gene-specific real-time RT-PCR.

引用

页码：2985 / 2988

页数：4

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