Gene cloning and expression of new trehalose-producing enzymes from the hyperthermophilic archaeum Sulfolobus solfataricus KM1

被引：29

作者：

Kobayashi, K ^{[1
]}

Kato, M ^{[1
]}

Miura, Y ^{[1
]}

Kettoku, M ^{[1
]}

Komeda, T ^{[1
]}

Iwamatsu, A ^{[1
]}

机构：

[1] KIRIN BREWERY CO LTD, CENT LABS KEY TECHNOL, KANAZAWA KU, YOKOHAMA, KANAGAWA 236, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 11期

关键词：

trehalose; Sulfolobus; glycosyltransferase; glycosyltrehalose; alpha-amylase family;

D O I：

10.1271/bbb.60.1882

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genes encoding for trehalose-producing enzymes, a glycosyl-trehalose-producing enzyme (glycosyltransferase) and a glycosyl-trehalose-hydrolyzing enzyme (alpha-amylase), from Sulfolobus sol-fataricus KM1 were cloned and expressed in E. coli. The nucleotide sequence of the glycosyltransferase gene and the alpha-amylase gene indicated proteins with lengths of 728 and 558 amino acids and molecular masses of 86-kDa and 65-kDa, respectively. Regions highly conserved in the alpha-amylase family exist in the amino acid sequences of these enzymes.

引用

页码：1882 / 1885

页数：4

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