Functional cytochrome oxidase histochemistry in the honeybee brain

被引：21

作者：

Armengaud, C ^{[1
]}

Causse, N ^{[1
]}

Aït-Oubah, J ^{[1
]}

Ginolhac, A ^{[1
]}

Gauthier, M ^{[1
]}

机构：

[1] Univ Toulouse 3, Lab Neurobiol Insecte, F-31062 Toulouse, France

来源：

BRAIN RESEARCH | 2000年 / 859卷 / 02期

关键词：

honeybee; cytochrome oxidase; histochemistry; nicotinic receptor; imidacloprid;

D O I：

10.1016/S0006-8993(00)02016-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The variations of neural metabolism induced by surgical and chemical treatments were studied in the honeybee brain by the means of cytochrome oxidase (CO) histochemistry. CO staining is considerably reduced in the alpha-lobe by antennal input deprivation. Chemical stimulation (50 mM K+) was linked to an increase of CO staining in antennal lobes (AL) and to a decrease in the basal ring of calyces (Cal). Application of the nicotinic ligand imidacloprid (10(-4) M) resulted in increased CO labelling within 30 min in all the structures analysed. Treatment with a lower concentration (10(-8) M) resulted in reduced staining in Cal and central body (CB). We conclude that CO histochemistry can be used to identify the target structures of cholinergic ligands in the honeybee brain. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：390 / 393

页数：4

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