Isolation and characterization of a cDNA from Catharanthus roseus which is highly homologous with phosphate transporter

被引:42
作者
Kai, M
Masuda, Y
Kikuchi, Y
Osaki, M
Tadano, T
机构
[1] KYOWA HAKKO KOGYO CO LTD,TSUKUBA RES LAB,TSUKUBA,IBARAKI 305,JAPAN
[2] HOKKAIDO UNIV,FAC AGR,SAPPORO,HOKKAIDO 060,JAPAN
关键词
Catharanthus roseus; human glucose transporter superfamily; hydrophobic protein; phosphate transporter; phosphate uptake system;
D O I
10.1080/00380768.1997.10414730
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The PIT1 gene which is highly homologous with phosphate transporter was isolated from Catharanthus roseus and analyzed, The cDNA PIT1 contained an open reading frame of 542 amino acids and its sequence showed a 31, 30, and 34% identity with the phosphate transporter of Saccharomyces cerevisiae (PH084), Neurospora crassa (PHO-S), and Glomus versiforme (GvPT), respectively, Furthermore, the cDNA PIT1 encoded a highly hydrophobic protein with 12 putative membrane-spanning regions and contained a conserved amino acid sequence reported in the human glucose transporter superfamily, S, cerevisiae strain DpU (pho84 knockout strain) was unable to grow on low phosphate (55 mu M) medium (LP medium), Expression of the PIT1 cDNA enabled DpU to grow on LP medium, Northern hybridization analysis revealed that the PIT1 gene was expressed in roots, stems, and young whole plant of C, roseus, but not in leaves.
引用
收藏
页码:227 / 235
页数:9
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