Inhibition of human neutrophil beta(2)-integrin-dependent adherence by hyperbaric O-2

被引:132
作者
Thom, SR
Mendiguren, I
Hardy, K
Bolotin, T
Fisher, D
Nebolon, M
Kilpatrick, L
机构
[1] UNIV PENN, DEPT EMERGENCY MED, PHILADELPHIA, PA 19104 USA
[2] UNIV PENN, DIV PULM & CRIT CARE MED, PHILADELPHIA, PA 19104 USA
[3] CHILDRENS HOSP, DEPT IMMUNOL, PHILADELPHIA, PA 19104 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1997年 / 272卷 / 03期
关键词
guanosine; 3'; 5'-cyclic monophosphate; neutrophil activation; guanylate cyclase; atrial natriuretic peptide; oxidative burst; fibrinogen;
D O I
10.1152/ajpcell.1997.272.3.C770
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Animal and clinical investigations have reported that exposure to hyperbaric O-2 improved the outcome of some reperfusion injuries. Animal studies have suggested that this may be due to an inhibition of leukocyte adherence to injured endothelium. This investigation tested the hypothesis that exposure to hyperbaric O-2 would inhibit beta(2)-integrin-dependent adherence of human neutrophils. Subjects were exposed to O-2 at partial pressures of up to 3 atmospheres absolute (ATA; 1 ATA = 0.1 MPa) for 45 min, and neutrophil binding to nylon columns and to fibrinogen-coated surfaces was measured. Exposure to O-2 at 2.8 or 3.0 ATA inhibited beta(2)-integrin-dependent neutrophil adherence but had no effect on the cell-surface expression of beta(2)-integrins, respiratory burst in response to phorbol ester, or non-beta(2)-integrin-dependent adherence to plastic plates coated with a fibronectin-like protein. beta(2)-Integrin adherence was restored by incubating blood with 8-bromoguanosine 3',5'-cyclic monophosphate (cGMP) and hyperbaric O-2 inhibited synthesis of cGMP by neutrophils stimulated with N-formyl-Met-Leu-Phe (FMLP). In studies of cell fractions, the activity of membrane guanylate cyclase was found to be increased by incubation with FMLP as well as by atrial natriuretic peptide (ANP) plus ATP. Hyperbaric O-2 had no effect on the basal activity of soluble or membrane-bound guanylate cyclase. However, hyperbaric O-2 inhibited the function of both the extracellular binding domain of membrane guanylate cyclase as well as intracellular catalytic activity. There are similar to 7,300 membrane guanylate cyclase molecules per cell, based on binding studies with ANP, with a dissociation constant of similar to 450 phl. Hyperbaric O-2 inhibits the function of human neutrophil beta(2)-integrins by a process linked to impaired synthesis of cGMP.
引用
收藏
页码:C770 / C777
页数:8
相关论文
共 34 条
[1]  
ANDERSON DC, 1987, ANNU REV MED, V38, P175, DOI 10.1146/annurev.med.38.1.175
[2]  
BERRY N, 1989, J IMMUNOL, V143, P1407
[3]  
BITTERMAN N, 1993, UNDERSEA HYPERBAR M, V20, P197
[4]   DIFFERENTIAL ACTIVATION BY ATRIAL AND BRAIN NATRIURETIC PEPTIDES OF 2 DIFFERENT RECEPTOR GUANYLATE CYCLASES [J].
CHANG, M ;
LOWE, DG ;
LEWIS, M ;
HELLMISS, R ;
CHEN, E ;
GOEDDEL, DV .
NATURE, 1989, 341 (6237) :68-72
[5]  
Chen QP, 1996, J PHARMACOL EXP THER, V276, P929
[6]  
Davis JC, 1988, PROBLEM WOUNDS ROLE, P225
[7]   AGGREGATION OF COMPLEMENT RECEPTORS ON HUMAN-NEUTROPHILS IN THE ABSENCE OF LIGAND [J].
DETMERS, PA ;
WRIGHT, SD ;
OLSEN, E ;
KIMBALL, B ;
COHN, ZA .
JOURNAL OF CELL BIOLOGY, 1987, 105 (03) :1137-1145
[8]  
DUCASSE JL, 1995, UNDERSEA HYPERBAR M, V22, P9
[9]  
ELLESTAD MH, 1992, CIRCULATION, V86, P47
[10]   CYCLIC GMP AND CELL MOVEMENT [J].
ESTENSEN, RD ;
HILL, HR ;
QUIE, PG ;
HOGAN, N ;
GOLDBERG, ND .
NATURE, 1973, 245 (5426) :458-460