Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs

被引：50

作者：

Nakamura, T

Ohta, M

Sugiura, M

Sugita, M ^{[1
]}

机构：

[1] Nagoya Univ, Ctr Gene Res, Nagoya, Aichi 4648601, Japan

[2] Nagoya Univ, Grad Sch Human Informat, Nagoya, Aichi 4648601, Japan

来源：

FEBS LETTERS | 1999年 / 460卷 / 03期

关键词：

chloroplast ribonucleoprotein; RNA processing; splicing; tobacco;

D O I：

10.1016/S0014-5793(99)01390-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tobacco chloroplasts possess five conserved ribonucleoproteins (cpRNPs). To elucidate the function of cpRNPs me analyzed their localization and target nucleic acid molecules in chloroplasts, Immunoprecipitation of the stromal extract and Northern analysis revealed that cpRNPs are associated in vivo with not only various species of chloroplast mRNAs but also intron-containing precursor (pre-) tRNAs. This observation strongly suggests that cpRNPs are involved in RNA processing, including mRNA stability and pre-tRNA splicing, (C) 1999 Federation of European Biochemical Societies.

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页码：437 / 441

页数：5

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