Identification and characterization of a gene encoding a vertebrate-type carbonic anhydrase in cyanobacteria

被引:93
作者
SoltesRak, E
Mulligan, ME
Coleman, JR
机构
[1] UNIV TORONTO,DEPT BOT,TORONTO,ON M5S 3B2,CANADA
[2] MEM UNIV NEWFOUNDLAND,DEPT BIOCHEM,ST JOHNS,NF,CANADA
关键词
D O I
10.1128/jb.179.3.769-774.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A gene (designated ecaA) encoding a vertebrate-like (alpha-type) carbonic anhydrase (CA) has been isolated from two disparate cyanobacteria, Anabaena sp. strain PCC 7120 and Synechococcus sp. strain PCC 7942. The deduced amino acid sequences correspond to proteins of 29 and 26 kDa, respectively, and revealed significant sequence similarity to human CAI and CAII, as well as Chlamydomonas CAHI, including conservation of most active-site residues identified in the animal enzymes, Structural similarities between the animal and cyanobacterial enzymes extend to the levels of antigenicity, as the Anabaena protein cross-reacts with antisera derived against chicken CAII. Expression of the cyanobacterial ecaA is regulated by CO2 concentration and is highest in cells grown at elevated levels of CO2. Immunogold localization using an antibody derived against the ecaA protein indicated an extracellular location. Preliminary analysis of Synechococcus mutants in which ecaA has been inactivated by insertion of a drug resistance cassette suggests that extracellular carbonic anhydrase plays a role in inorganic-carbon accumulation by maintaining equilibrium levels of CO2 and HCO3- in the periplasm.
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收藏
页码:769 / 774
页数:6
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