Purification and partial characterization of a laccase from the white rot fungus Phanerochaete flavido-alba

被引：31

作者：

Perez, J ^{[1
]}

Martinez, J ^{[1
]}

delaRubia, T ^{[1
]}

机构：

[1] UNIV GRANADA,FAC FARM,DEPT MICROBIOL,E-18071 GRANADA,SPAIN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 11期

关键词：

D O I：

10.1128/AEM.62.11.4263-4267.1996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In addition to excreting lignin-degrading peroxidases, the white rot fungus Phanerochaete flavido-alba also excretes a laccase. This protein was purified to homogeneity and found to have a molecular weight of 94,000 and an isoelectric point lower than 3.55. Its UV-visible spectrum is typical of copper-containing proteins.

引用

页码：4263 / 4267

页数：5

共 37 条

[1] COMPARATIVE STUDIES OF EXTRACELLULAR FUNGAL LACCASES [J].

BOLLAG, JM ;

LEONOWICZ, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 48 (04) :849-854

[2] MN(II) REGULATION OF LIGNIN PEROXIDASES AND MANGANESE-DEPENDENT PEROXIDASES FROM LIGNIN-DEGRADING WHITE ROT FUNGI [J].

BONNARME, P ;

JEFFRIES, TW .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (01) :210-217

[3] LIGNIN BIODEGRADATION [J].

BUSWELL, JA ;

ODIER, E .

CRC CRITICAL REVIEWS IN BIOTECHNOLOGY, 1987, 6 (01) :1-60

[4] CHARACTERIZATION AND STRUCTURAL-ANALYSIS OF THE LACCASE-I GENE FROM THE NEWLY ISOLATED LIGNINOLYTIC BASIDIOMYCETE PM1 (CECT 2971) [J].

COLL, PM ;

TABERNERO, C ;

SANTAMARIA, R ;

PEREZ, P .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (12) :4129-4135

[5] PURIFICATION AND CHARACTERIZATION OF A PHENOLOXIDASE (LACCASE) FROM THE LIGNIN-DEGRADING BASIDIOMYCETE PM1 (CECT-2971) [J].

COLL, PM ;

FERNANDEZABALOS, JM ;

VILLANUEVA, JR ;

SANTAMARIA, R ;

PEREZ, P .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (08) :2607-2613

[6]

Eriksson K.E., 1990, MICROBIAL ENZYMATIC, P225

[7] LIGNIN DEGRADATION BY RIGIDOPORUS-LIGNOSUS INVOLVES SYNERGISTIC ACTION OF 2 OXIDIZING ENZYMES - MN PEROXIDASE AND LACCASE [J].

GALLIANO, H ;

GAS, G ;

SERIS, JL ;

BOUDET, AM .

ENZYME AND MICROBIAL TECHNOLOGY, 1991, 13 (06) :478-482

[8]

GALLIANO H, 1988, PLANT PHYSIOL BIOCH, V26, P619

[9]

GOLD MH, 1989, ACS SYM SER, V389, P127

[10]

GUILLEN F, 1990, APPL MICROBIOL BIOT, V32, P469

← 1 2 3 4 →