Identification and sequencing of bacterial rDNAs in culture-negative amniotic fluid from women in premature labor

被引:88
作者
Gardella, C
Riley, DE
Hitti, J
Agnew, K
Krieger, JN
Eschenbach, D
机构
[1] Univ Washington, Med Ctr, Dept Obstet & Gynecol, Seattle, WA 98195 USA
[2] Univ Washington, Med Ctr, Dept Urol, Seattle, WA 98195 USA
[3] VA Med Ctr, Seattle, WA USA
关键词
16S rDNA; PCB; preterm labor; amniotic fluid; intra-amniotic infection;
D O I
10.1055/s-2004-831884
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Our objective was to identify bacterial species present in culture-negative but 16S rDNA-positive amniotic fluid samples from women in preterm labor. Amniotic fluid from 69 women in preterm labor was cultured and examined for the proinflammatory cytokine interleukin-6 (IL-6). Polymerase chain reaction technology was used to detect highly conserved bacterial ribosomal DNA sequences (16S rDNAs). As previously reported, 16S rDNAs were identified in 15 (94%) of 16 culture-positive amniotic fluid samples, in 5 (36%) of 14 culture-negative samples with elevated IL-6, and in 1 (3%) of 39 culture-negative samples with low IL-6 levels. Direct sequencing was performed of 16S rDNAs from the 5 culture-negative amniotic fluid specimens with elevated IL-6, followed by database searches and phylogenetic analyses. The bacterial sequences identified included: two LePtotrichia sanguinegens, one human oral bacterium A33, one Fusobacterium nucleatum, and one Ureaplasma urealyticum. Identification and sequencing of 16S rDNAs in amniotic fluid is a promising technique to identify bacterial species associated with elevated IL-6 levels in culture- negative amniotic fluid that may contribute to the etiology of premature labor.
引用
收藏
页码:319 / 323
页数:5
相关论文
共 16 条
[1]   Possible association between amniotic fluid micro-organism infection and microflora in the mouth [J].
Bearfield, C ;
Davenport, ES ;
Sivapathasundaram, V ;
Allaker, RP .
BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 2002, 109 (05) :527-533
[2]   Characterization of some strains from human clinical sources which resemble "Leptotrichia sanguinegens":: Description of Sneathia sanguinegens sp nov., gen. nov. [J].
Collins, MD ;
Hoyles, L ;
Törnqvist, E ;
von Essen, R ;
Falsen, E .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2001, 24 (03) :358-361
[3]   Amniotic fluid lipopolysaccharide-binding protein and soluble CD14 as mediators of the inflammatory response in preterm labor [J].
Gardella, C ;
Hitti, J ;
Martin, TR ;
Ruzinski, JT ;
Eschenbach, D .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2001, 184 (06) :1241-1248
[4]   LEPTOTRICHIA SANGUINEGENS SP-NOV, A NEW AGENT OF POSTPARTUM AND NEONATAL BACTEREMIA [J].
HANFF, PA ;
ROSOLDONOGHUE, JA ;
SPIEGEL, CA ;
WILSON, KH ;
MOORE, LH .
CLINICAL INFECTIOUS DISEASES, 1995, 20 :S237-S239
[5]  
HILLIER SL, 1993, OBSTET GYNECOL, V81, P941
[6]   Broad-spectrum bacterial rDNA polymerase chain reaction assay for detecting amniotic fluid infection among women in premature labor [J].
Hitti, J ;
Riley, DE ;
Krohn, MA ;
Hillier, SL ;
Agnew, KJ ;
Krieger, JN ;
Eschenbach, DA .
CLINICAL INFECTIOUS DISEASES, 1997, 24 (06) :1228-1232
[7]   Vaginal indicators of amniotic fluid infection in preterm labor [J].
Hitti, J ;
Hillier, SL ;
Agnew, KJ ;
Krohn, MA ;
Reisner, DP ;
Eschenbach, DA .
OBSTETRICS AND GYNECOLOGY, 2001, 97 (02) :211-219
[8]  
McGaw Tim, 2002, J Can Dent Assoc, V68, P165
[9]   Does blood of healthy subjects contain bacterial ribosomal DNA? [J].
Nikkari, S ;
McLaughlin, IJ ;
Bi, WL ;
Dodge, DE ;
Relman, DA .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (05) :1956-1959
[10]  
Palmer H M, 1991, Int J STD AIDS, V2, P261