Identification of a cellular ligand for the natural cytotoxicity receptor NKp44

被引:137
作者
Baychelier, Florence [1 ,2 ]
Sennepin, Alexis [1 ,2 ]
Ermonval, Myriam [3 ]
Dorgham, Karim [1 ,2 ]
Debre, Patrice [1 ,2 ,4 ]
Vieillard, Vincent [1 ,2 ]
机构
[1] INSERM, UMR S945, Lab Immunite & Infect, Paris, France
[2] Univ Paris 06, UMR S945, Lab Immunite & Infect, Paris, France
[3] Inst Pasteur, Dept Virol, Paris, France
[4] Hop La Pitie Salpetriere, AP HP, Dept Immunol, Paris, France
关键词
CD4(+) T-CELLS; KILLER-CELLS; ACTIVATING RECEPTOR; HUMAN CYTOMEGALOVIRUS; VIRUS HEMAGGLUTININ; CYCLE PROGRESSION; IMMUNE-RESPONSES; NKG2D LIGANDS; NK CELLS; EXPRESSION;
D O I
10.1182/blood-2013-03-489054
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
With an array of activating and inhibitory receptors, natural killer (NK) cells are involved in the eradication of infected, transformed, and tumor cells. NKp44 is a member of the natural cytotoxicity receptor family, which is exclusively expressed on activated NK cells. Here, we identify natural cytotoxicity receptor NKp44 (NKp44L), a novel isoform of the mixed-lineage leukemia-5 protein, as a cellular ligand for NKp44. Unlike the other MLL family members, NKp44L is excluded from the nucleus, but expressed at the cell-surface level; its subcellular localization is being associated with the presence of a specific C-terminal motif. Strikingly, NKp44L has not been detected on circulating cells isolated from healthy individuals, but it is expressed on a large panel of the tumor and transformed cells. The sharply decreased NK lysis activity induced by anti-NKp44L antibodies directly demonstrates the role of NKp44L in cytotoxicity. Taken together, these results show that NKp44L could be critical for NK cell-mediated innate immunity. The identification and cellular distribution of NKp44L highlight the role of this self-molecule as a danger signal to alert the NK cell network.
引用
收藏
页码:2935 / 2942
页数:8
相关论文
共 47 条
[1]   Molecular basis for chromatin binding and regulation of MLL5 [J].
Ali, Muzaffar ;
Rincon-Arano, Hector ;
Zhao, Wei ;
Rothbart, Scott B. ;
Tong, Qiong ;
Parkhurst, Susan M. ;
Strahl, Brian D. ;
Deng, Lih-Wen ;
Groudine, Mark ;
Kutateladze, Tatiana G. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (28) :11296-11301
[2]   Inhibition of the NKp30 activating receptor by pp65 of human cytomegalovirus [J].
Arnon, TI ;
Achdout, H ;
Levi, O ;
Markel, G ;
Saleh, N ;
Katz, G ;
Gazit, R ;
Gonen-Gross, T ;
Hanna, J ;
Nahari, E ;
Porgador, A ;
Honigman, A ;
Plachter, B ;
Mevorach, D ;
Wolf, DG ;
Mandelboim, O .
NATURE IMMUNOLOGY, 2005, 6 (05) :515-523
[3]   Involvement of the Gab2 scaffolding adapter in type I interferon signalling [J].
Baychelier, Florence ;
Nardeux, Pierre-Claude ;
Cajean-Feroldi, Chantal ;
Ermonval, Myriam ;
Guymarho, Jacqueline ;
Tovey, Michaeel G. ;
Eid, Pierre .
CELLULAR SIGNALLING, 2007, 19 (10) :2080-2087
[4]   The B7 family member B7-H6 is a tumor cell ligand for the activating natural killer cell receptor NKp30 in humans [J].
Brandt, Cameron S. ;
Baratin, Myriam ;
Yi, Eugene C. ;
Kennedy, Jacob ;
Gao, Zeren ;
Fox, Brian ;
Haldeman, Betty ;
Ostrander, Craig D. ;
Kaifu, Tomonori ;
Chabannon, Christian ;
Moretta, Alessandro ;
West, Robert ;
Xu, WenFeng ;
Vivier, Eric ;
Levin, Steven D. .
JOURNAL OF EXPERIMENTAL MEDICINE, 2009, 206 (07) :1495-1503
[5]   Expression Analysis of the Ligands for the Natural Killer Cell Receptors NKp30 and NKp44 [J].
Byrd, Andreina ;
Hoffmann, Sabrina C. ;
Jarahian, Mostafa ;
Momburg, Frank ;
Watzl, Carsten .
PLOS ONE, 2007, 2 (12)
[6]   Human natural killer cells [J].
Caligiuri, Michael A. .
BLOOD, 2008, 112 (03) :461-469
[7]   The three-dimensional structure of the human NK cell receptor NKp44, a triggering partner in natural cytotoxicity [J].
Cantoni, C ;
Ponassi, M ;
Biassoni, R ;
Conte, R ;
Spallarossa, A ;
Moretta, A ;
Moretta, L ;
Bolognesi, M ;
Bordo, D .
STRUCTURE, 2003, 11 (06) :725-734
[8]   NKp44, a triggering receptor involved in tumor cell lysis by activated human natural killer cells, is a novel member of the immunoglobulin superfamily [J].
Cantoni, C ;
Bottino, C ;
Vitale, M ;
Pessino, A ;
Augugliaro, R ;
Malaspina, A ;
Parolini, S ;
Moretta, L ;
Moretta, A ;
Biassoni, R .
JOURNAL OF EXPERIMENTAL MEDICINE, 1999, 189 (05) :787-795
[9]   Expansion of human NK-22 cells with IL-7, IL-2, and IL-1β reveals intrinsic functional plasticity [J].
Cella, Marina ;
Otero, Karel ;
Colonna, Marco .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (24) :10961-10966
[10]   NKG2D ligands: unconventional MHC class I-like molecules exploited by viruses and cancer [J].
Cerwenka, A ;
Lanier, LL .
TISSUE ANTIGENS, 2003, 61 (05) :335-343