Membrane topology of the ZntB efflux system of Salmonella enterica serovar typhimurium

被引:19
作者
Caldwell, AM [1 ]
Smith, RL [1 ]
机构
[1] Univ Texas, Dept Biol, Arlington, TX 76019 USA
关键词
D O I
10.1128/JB.185.1.374-376.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The membrane topology of the ZntB Zn2+ transport protein of Salmonella enterica serovar Typhimurium was determined by constructing deletion derivatives of the protein and genetically fusing them to blaM or lacZ cassettes. The enzymatic activities of the hybrid proteins indicate that ZntB is a bitopic integral membrane protein consisting largely of two independent domains. The first 266 amino acids form a large, highly charged domain within the cytoplasm, while the remaining 61 residues form a small membrane domain containing two membrane-spanning segments. The overall orientation towards the cytoplasm is consistent with the ability of ZntB to facilitate zinc efflux.
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页码:374 / 376
页数:3
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