Stat3-mediated transformation of NIH-3T3 cells by the constitutively active Q205L Gαo protein

被引:95
作者
Ram, PT
Horvath, CM
Iyengar, R
机构
[1] Mt Sinai Sch Med, Dept Pharmacol, New York, NY 10029 USA
[2] Mt Sinai Sch Med, Immunobiol Ctr, New York, NY 10029 USA
关键词
D O I
10.1126/science.287.5450.142
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Expression of Q205L G alpha(o) (G alpha(o)*), an alpha subunit of heterotrimeric guanine nucleotide-binding proteins (G proteins) that lacks guanosine triphosphatase (GTPase) activity in NIH-3T3 cells, results in transformation. Expression of G alpha(o)* in NIH-3T3 cells activated signal transducer and activator of transcription 3 (Stat3) but not mitogen-activated protein (MAP) kinases 1 or 2, Coexpression of dominant negative Stat3 inhibited G alpha(o)*-induced transformation of NIH-3T3 cells and activation of endogenous Stat3, Furthermore, G alpha(o)* expression increased activity of the tyrosine kinase c-Src, and the G alpha(o)*-induced activation of Stat3 was blocked by expression of Csk (carboxyl-terminal Src kinase), which inactivates c-Src. The results indicate that Stat3 can function as a downstream effector for G alpha(o)* and mediate its biological effects.
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页码:142 / 144
页数:3
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