Innate murine B cells produce anti-disialosyl antibodies reactive with Campylobacter jejuni LPS and gangliosides that are polyreactive and encoded by a restricted set of unmutated V genes

被引：25

作者：

Boffey, J

Nicholl, D

Wagner, ER

Townson, K

Goodyear, C

Furukawa, K

Conner, J

Willison, HJ ^{[1
]}

机构：

[1] Univ Glasgow, So Gen Hosp, Inst Neurol Sci, Dept Neurol, Glasgow G51 4TF, Lanark, Scotland

[2] Glasgow Caledonian Univ, Dept Sci Biol, Glasgow G4 0BA, Lanark, Scotland

[3] Nagoya Univ, Sch Med, Dept Biochem 2, Nagoya, Aichi 466, Japan

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2004年 / 152卷 / 1-2期

关键词：

autoantibodies; gangliosides; lipopolysaccharide; neuropathy; V gene usage; innate immunity;

D O I：

10.1016/j.jneuroim.2004.04.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In Guillain-Barre syndrome following Campylobacter enteritis, anti-lipopolysaccharide antibodies cross-react with neural gangliosides, thereby precipitating autoimmune neuropathy. We examined the properties of 15 murine anti-LPS/ganglioside mAbs specific for NeuAc(alpha2-8)NeuAc-Gal disialosyl epitopes. Many mAbs displayed features of an innate B cell origin including polyreactivity (13/15), hybridoma CD5 mRNA expression (5115), predominance of IgM (9/15) or IgG3 (3/6) isotype, low affinity, and utilisation of unmutated V-H and V-L V(D)J rearrangements. Antibody specificity resided in highly selective V gene usage, with 6/15 mAbs being encoded by the VH7183.3b gene. These data indicate that neuropathogenic antiganglioside autoantibodies can arise from the natural autoantibody repertoire. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：98 / 111

页数：14

共 59 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].