Relationship between the glutamate production and the activity of 2-oxoglutarate dehydrogenase in Brevibacterium lactofermentum

被引:81
作者
Kawahara, Y
TakahashiFuke, K
Shimizu, E
Nakamatsu, T
Nakamori, S
机构
[1] Technology and Engineering Laboratories of Ajinomoto Co, Inc, Kawasaki, 210, 1-1 Suzuki-cho, Kawasaki-ku
[2] Department of Bioscience, Fukui Prefectural University, Fukui, 910-11, 4-1-1 Kenjyojima, Matsuoka-cho
关键词
glutamate production; Brevibacterium lactofermentum; 2-oxoglutarate dehydrogenase;
D O I
10.1271/bbb.61.1109
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Enzyme activities of 2-oxoglutarate dehydrogenase complex and glutamate dehydrogenase of wild type Brevibacterium lactofermentum, one of the typical glutamate-producing coryneform bacteria, were investigated by using cells cultured under glutamate-productive and glutamate-non-productive conditions. Significant reduction of the former enzyme activity was observed in the cells under the several glutamate-productive conditions, namely, in the cells cultured in media containing a) limited concentrations of biotin, b) sub-lethal amounts of penicillin, and c) sub-optimal amounts of a surface-active agent, as compared with those under the non-productive conditions, The activity of the latter enzyme was essentially unchanged in every condition, The relationship between glutamate production and the enzyme activities as well as permeability of glutamate through cell membrane was discussed from the results obtained.
引用
收藏
页码:1109 / 1112
页数:4
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