A combination of diagnostic tools for rapid screening of ovine listeriosis

被引：10

作者：

Amagliani, G.

Giammarini, C.

Omiccioli, E.

Merati, E. G.

Pezzotti, G.

Filippini, G.

Brandi, G.

Magnani, M.

机构：

[1] Univ Urbino, Ctr Biotecnol, I-61032 Fano, PU, Italy

[2] Univ Urbino, Ist Sci Tossic Igienist & Ambientali, I-61032 Fano, PU, Italy

[3] Diatheva Srl, I-61032 Fano, PU, Italy

[4] Ist Zooprofilatt Sperimentale Umbria & Marche, I-06126 Perugia, Italy

[5] Univ Urbino, Ist Chim Biol G Fornaini, I-61029 Urbino, PU, Italy

来源：

RESEARCH IN VETERINARY SCIENCE | 2006年 / 81卷 / 02期

关键词：

PCR; ELISA; ovine listeriosis; Listeria monocytogenes; listeriolysin O; epidemiological survey;

D O I：

10.1016/j.rvsc.2005.12.006

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A combined serological and PCR method for the detection of Listeria nionocytogenes infection in symptomatic and asymptornatic ovine flocks was evaluated. Seventy-eight milk samples and 157 serum samples were analysed using a L. monocytogenes PCR detection kit and an anti-listeriolysin O IgG immunoassay kit. The combined use of these commercial kits allowed a rapid and effective detection of L. monocytogenes infection in both the early stage, before seroconversion, and in a later phase, even after antibiotic therapy. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：185 / 189

页数：5

共 27 条

[1] Direct detection of Listeria monocytogenes from milk by magnetic based DNA isolation and PCR [J].