Enzymatic synthesis of UDP-galactose on a gram scale

被引：16

作者：

Bülter, T ^{[1
]}

Elling, L ^{[1
]}

机构：

[1] Univ Dusseldorf, Inst Enzyme Technol, Res Ctr Julich, D-52426 Julich, Germany

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2000年 / 8卷 / 4-6期

关键词：

enzymatic synthesis; UDP-galactose; nucleotide sugars; repetitive batch;

D O I：

10.1016/S1381-1177(99)00079-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uridine 5'-diphospho-alpha-D-galactose (UDP-Gal) was synthesized on a gram scale from uridine 5'-diphospho-alpha-D-glucose and cr-D-galactose l-phosphate using the enzymes galactose-l-phosphate uridyltransferase (EC 2.7.7.12), phosphoglucomutase (EC 2.7.5.1) and glucose-6-phosphate dehydrogenase (EC 1.1.1.27). The synthesis was performed in a repetitive batch mode in which the enzymes, some of which are expensive, were used in 16 subsequent batches without any loss of enzyme activity. The space time yield of the synthesis was 7.1 g/l d. The overall yield of the synthesis amounted to 40% and 1.1 gram of pure UDP-Gal was obtained. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：281 / 284

页数：4

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