Nucleic acid 3′-end recognition by the Argonaute2 PAZ domain

被引：238

作者：

Lingel, A ^{[1
]}

Simon, B ^{[1
]}

Izaurralde, E ^{[1
]}

Sattler, M ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2004年 / 11卷 / 06期

关键词：

D O I：

10.1038/nsmb777

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides. The structures reveal a unique mode of single-stranded nucleic acid binding in which the two 3'-terminal nucleotides are buried in a hydrophobic cleft. We propose that the PAZ domain contributes to the specific recognition of siRNAs by providing a binding pocket for their characteristic two-nucleotide 3' overhangs.

引用

页码：576 / 577

页数：2

共 6 条

[1] The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis [J].