Comprehensive genome and transcriptome analysis of the 11q13 amplicon in human oral cancer and synteny to the 7F5 amplicon in murine oral carcinoma

被引:115
作者
Huang, Xin
Godfrey, Tony E.
Gooding, William E.
McCarty, Kenneth S., Jr.
Gollin, Susanne M.
机构
[1] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Human Genet, Pittsburgh, PA 15261 USA
[2] Univ Pittsburgh, Inst Canc, Pittsburgh, PA 15232 USA
[3] Univ Pittsburgh, Sch Med, Dept Surg, Pittsburgh, PA 15261 USA
[4] Univ Pittsburgh, Sch Med, Dept Pathol, Pittsburgh, PA 15261 USA
[5] Univ Pittsburgh, Sch Med, Dept Med, Pittsburgh, PA 15261 USA
关键词
D O I
10.1002/gcc.20371
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
11q13 amplification occurs in a wide variety of tumors, including almost half of oral squamous cell carcinomas (OSCC) where it has been correlated with a poor outcome. In this study, we compiled 3.6 Mb of DNA sequence in the 11q13 amplicon core and refined the physical map of the amplicon. In the process, we determined the genomic structure and normal tissue expression patterns of two recently identified genes, TAOS2/TMEM16A and MRGF, which reside in the amplicon core. We then quantified DNA copy number and mRNA expression of all genes in the 11q13 amplicon in cell lines and primary tumors from OSCC. With the exception of FGF3, FGF4, FGF19, and MRGF, all genes were overexpressed in most tumors with genomic amplification. Furthermore, we found that the expression of genes in the amplicon appeared to be highly coordinated, making it difficult to determine which gene or genes are driving amplification. However, in nonamplified primary tumors, three genes, TAOS2/TMEM16A, OC1M, and TPCN2, are frequently overexpressed, whereas CCND1 and EMS 1 are not. These results suggest that in addition to CCND1 and EMS1, other important genes also may be target genes driving 11q13 amplification. We hypothesize that 11q13 amplification may be driven by a cassette of genes that provide growth or metastatic advantage to cancer cells. This is supported by the finding that the human 11q13 amplicon core is syntenic to mouse chromosomal band 7F5, which is frequently amplified in chemically induced murine OSCC. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat. (c) 2006 Wiley-Liss, Inc.
引用
收藏
页码:1058 / 1069
页数:12
相关论文
共 57 条
[1]  
Akervall JA, 1997, CANCER, V79, P380
[2]  
AKERVALL JA, 1995, CANCER, V76, P853, DOI 10.1002/1097-0142(19950901)76:5<853::AID-CNCR2820760520>3.0.CO
[3]  
2-6
[4]   Detailed map of a region commonly amplified at 11q13→q14 in human breast carcinoma [J].
Bekri, S ;
Adélaïde, J ;
Merscher, S ;
Grosgeorge, J ;
Caroli-Bosc, F ;
Perucca-Lostanlen, D ;
Kelley, PM ;
Pébusque, MJ ;
Theillet, C ;
Birnbaum, D ;
Gaudray, P .
CYTOGENETICS AND CELL GENETICS, 1997, 79 (1-2) :125-131
[5]  
BERENSON JR, 1990, ONCOGENE, V5, P1343
[6]   Prognostic value of CCND1 gene status in sporadic breast tumours, as determined by real-time quantitative PCR assays [J].
Bièche, I ;
Olivi, M ;
Noguès, C ;
Vidaud, M ;
Lidereau, R .
BRITISH JOURNAL OF CANCER, 2002, 86 (04) :580-586
[7]   ASSOCIATION OF INT2/HST1 COAMPLIFICATION IN PRIMARY BREAST-CANCER WITH HORMONE-DEPENDENT PHENOTYPE AND POOR PROGNOSIS [J].
BORG, A ;
SIGURDSSON, H ;
CLARK, GM ;
FERNO, M ;
FUQUA, SAW ;
OLSSON, H ;
KILLANDER, D ;
MCGURIE, WL .
BRITISH JOURNAL OF CANCER, 1991, 63 (01) :136-142
[8]   Positional cloning of ZNF217 and NABC1:: Genes amplified at 20q13.2 and overexpressed in breast carcinoma [J].
Collins, C ;
Rommens, JM ;
Kowbel, D ;
Godfrey, T ;
Tanner, M ;
Hwang, S ;
Polikoff, D ;
Nonet, G ;
Cochran, J ;
Myambo, K ;
Jay, KE ;
Froula, J ;
Cloutier, T ;
Kuo, WL ;
Yaswen, P ;
Dairkee, S ;
Giovanola, J ;
Hutchinson, GB ;
Isola, J ;
Kallioniemi, OP ;
Palazzolo, M ;
Martin, C ;
Ericsson, C ;
Pinkel, D ;
Albertson, D ;
Li, WB ;
Gray, JW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (15) :8703-8708
[9]   Expression of fragile sites triggers intrachromosomal mammalian gene amplification and sets boundaries to early amplicons [J].
Coquelle, A ;
Pipiras, E ;
Toledo, F ;
Buttin, G ;
Debatisse, M .
CELL, 1997, 89 (02) :215-225
[10]   AMPLIFICATION OF CHROMOSOME BAND 11Q13 AND A ROLE FOR CYCLIN D1 IN HUMAN BREAST-CANCER [J].
DICKSON, C ;
FANTL, V ;
GILLETT, C ;
BROOKES, S ;
BARTEK, J ;
SMITH, R ;
FISHER, C ;
BARNES, D ;
PETERS, G .
CANCER LETTERS, 1995, 90 (01) :43-50