PCR-based assay to quantify human immunodeficiency virus type 1 DNA in peripheral blood mononuclear cells

An assay that quantifies the amount of human immunodeficiency virus type I (HIV-1) DNA in peripheral blood mononuclear cells has been developed, PCR amplification of the HIV-1 DNA is performed in the presence of an internal quantitation standard, and colorimetric detection of the amplified product is performed with micron-elf plates. The copies of HIV-1 DNA are normalized to total genomic DNA input. The assay has an analytical sensitivity of 10 input copies per amplification reaction and a three-log detection range, In an analysis of sequential samples from patients on combination therapy, HIV-1 DNA aas quantifiable for all individuals tested, including those with undetectable plasma HIV-1 RNA In a separate study, a comparison of HIV-1 DNA levels was made with a group of long-term survivors and progressors, The mean HIV-1 DNA levels were loner in the long-term survivors than in the progressors (P, 0.04). The mean HIV-1 RNA levels were also lower, but the difference was not statistically significant (P, 0.164). ri quantitative DNA assay will provide an additional tool to gain insight into the natural history of infection and the continued efficacy of potent antiretroviral therapies.