Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction

A total of 51 Vibrio minicus clinical strains from different geographic locations were examined by arbitrarily primed polymerase chain reaction (AP-PCR), The primer VMH-3 divided them into 28 groups, although 18 groups consisted of a single strain at present. All groups had a common 1.0-kb amplification fragment. Most of the groups consisted of strains from same region, although two exceptional groups showed a few amplification fragments including strains from different regions. AP-PCR groups were not consistently associated with serogroups, AP-PCR is thought to be a valuable and easy method for the epidemiological study of V. minicus.