Determination of cyclosporin A and metabolites total concentration using a molecularly imprinted polymer based radioligand binding assay.

被引：36

作者：

Senholdt, M ^{[1
]}

Siemann, M ^{[1
]}

Mosbach, K ^{[1
]}

Andersson, LI ^{[1
]}

机构：

[1] LUND UNIV,CTR CHEM,DEPT PURE & APPL BIOCHEM,S-22100 LUND,SWEDEN

来源：

ANALYTICAL LETTERS | 1997年 / 30卷 / 10期

关键词：

immunoassay like technique; predetermined selectivity; antibody binding site mimics; radioligand binding assay; polymeric sorbent;

D O I：

10.1080/00032719708001699

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Molecular imprinting of cyclosporin A was undertaken and the resultant polymer preparation was applied to the development of a radioligand binding assay for determination of the total concentration of cyclosporin A and its metabolites. The assay method comprised extraction of haemolyzed whole blood with diisopropyl ether followed by competitive radio-'immuno'-assay of the organic phase employing imprinted polymer particles and [H-3]cyclosporin. The assay measured the parent drug and the metabolites AM1, AM9 and AM4N with equal response. Concomitant analysis of patient samples using a specific EMIT assay in parallel with this novel imprinted polymer-based assay permitted simultaneous determination of the concentration of cyclosporin A and that of its metabolites.

引用

页码：1809 / 1821

页数：13

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