Optimization of Growth Medium and Enzyme Assay Conditions for Crude Cellulases Produced by a Novel Thermophilic and Cellulolytic Bacterium, Anoxybacillus sp 527

被引：52

作者：

Liang, Yanna ^{[1
]}

Feng, Zisong ^{[1
]}

Yesuf, Jemil ^{[1
]}

Blackburn, James W. ^{[2
]}

机构：

[1] So Illinois Univ, Dept Civil & Environm Engn, Carbondale, IL 62901 USA

[2] So Illinois Univ, Dept Mech Engn & Energy Proc, Carbondale, IL 62901 USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2010年 / 160卷 / 06期

关键词：

Cellulose; Thermophilic; Cellulase; Anoxybacillus sp 527; CLOSTRIDIUM-THERMOCELLUM; CULTURE-CONDITIONS; ETHANOL; HYDROLYSIS; ENDOGLUCANASE; PURIFICATION; SUBSTRATE; BIOMASS; FERMENTATION; DEGRADATION;

D O I：

10.1007/s12010-009-8677-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

A newly isolated Anoxybacillus sp. 527 was found to grow on crystalline cellulose as sole carbon and energy sources. Cellulases secreted by strain 527 were better induced by cellobiose, followed by glucose, lactose, sucrose, and cellulose. Cellulase secretion was enhanced by an optimized medium. Cellulase activity was increased by the addition of Ca(2+) and NH (4) (+) and achieved maximum as 7.0 FPU ml(-1) at 70 A degrees C and pH 6.0. Even at 100 A degrees C, the enzymes were still active, which implies their potential application in large-scale cellulose conversion process.

引用

页码：1841 / 1852

页数：12

共 50 条

[1]

Atlas R.M., 2004, HDB MICROBIOLOGICAL

[2]

EFFECTS OF THE SURFACTANT TWEEN-80 ON ENZYMATIC-HYDROLYSIS OF NEWSPAPER [J].

CASTANON, M ;

WILKE, CR .

BIOTECHNOLOGY AND BIOENGINEERING, 1981, 23 (06) :1365-1372

[3]

OPTIMIZATION OF CELLULASE PRODUCTION BY PENICILLIUM-OCCITANIS [J].

CHAABOUNI, SE ;

BELGUITH, H ;

HASSAIRI, I ;

MRAD, K ;

ELLOUZ, R .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1995, 43 (02) :267-269

[4]

FACTORS INFLUENCING PRODUCTION OF CELLULASES BY SPOROTRICHUM-THERMOPHILE [J].

COUTTS, AD ;

SMITH, RE .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1976, 31 (06) :819-825

[5]

Cellulase, clostridia, and ethanol [J].

Demain, AL ;

Newcomb, M ;

Wu, JHD .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2005, 69 (01) :124-+

[6]

Carbon flux distribution and kinetics of cellulose fermentation in steady-state continuous cultures of Clostridium cellulolyticum on a chemically defined medium [J].

Desvaux, M ;

Guedon, E ;

Petitdemange, H .

JOURNAL OF BACTERIOLOGY, 2001, 183 (01) :119-130

[7]

EUNKI K, 1998, BIOTECHNOL BIOENG, V60, P70, DOI DOI 10.1002/(SICI)1097-0290(19981005)60:1<70::AID-BIT8>3.0.C0

[8]

2-Y

[9]

Lactose Enhances Cellulase Production by the Filamentous Fungus Acremonium cellulolyticus [J].

Fang, Xu ;

Yano, Shinichi ;

Inoue, Hiroyuki ;

Sawayama, Shigeki .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2008, 106 (02) :115-120

[10]

PURIFICATION AND CHARACTERIZATION OF ENDOGLUCANASE-SS FROM CLOSTRIDIUM-THERMOCELLUM [J].

FAUTH, U ;

ROMANIEC, MPM ;

KOBAYASHI, T ;

DEMAIN, AL .

BIOCHEMICAL JOURNAL, 1991, 279 :67-73

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