T lymphocyte subpopulations and B lymphocyte cells in caecum and spleen of chicks infected with Salmonella enteritidis

被引:13
作者
Asheg, A [1 ]
Levkut, M [1 ]
Revajová, V [1 ]
Sevcíková, Z [1 ]
Kolodzieyski, L [1 ]
Pistl, J [1 ]
机构
[1] Univ Vet Med, Inst Pathol Anat, Kosice 04181, Slovakia
关键词
Salmonella enteritidis; local immunity; caecum; spleen; immunohistochemistry; flow cytometry; chicken;
D O I
10.1078/0065-1281-00666
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The effect of low and high doses of Salmonella enteritidis PT4 (SE) on immunocompetent cells in caecum and spleen of one-day-old chicks was investigated. Subsets of T lymphocytes positive for CD3, CD4, CD8 and B lymphocytes (Bulb-positive cells) were counted in the caecum after immunohistochemical staining and the relative percentage of these cells in the spleen was analysed using a FACScan cytometer on days 7, 10, 14, 21, and 27 post-inoculation (pi). In the low dose group, the number of CD3+ and CD4+ cells in the caecum had significantly increased at day 10 pi. Both CD8+ and Bulb+ cells were significantly higher on day 14 pi in this group. In the high-dose group, the number of CD4+ cells had significantly increased at day 7 pi. CD3+, CD8+, and BuIb+ cells showed prolonged proliferation at days 7 up to 21 pi. Splenic lymphocytes demonstrated significant changes only in the high dose group. The percentage of splenic CD4+ cells was decreased at day 7 pi. A decrease in CD3+ and CD8+ cells was found at day 14 pi in this group.
引用
收藏
页码:435 / 439
页数:5
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