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Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes

被引：30
作者：
Radwanska, M
Magez, S
Perry-O'Keefe, H
Stender, H
Coull, J
Sternberg, JM
Büscher, P
Hyldig-Nielsen, JJ
机构：
[1] Inst Trop Med, Dept Parasitol, Antwerp, Belgium
[2] Free Univ Brussels VIB, Cellular Immunol Lab, Brussels, Belgium
[3] Univ Aberdeen, Aberdeen, Scotland
[4] Appl Biosyst Inc, Bedford, MA USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 11期
关键词：
D O I：
10.1128/JCM.40.11.4295-4297.2002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.
引用
收藏
页码：4295 / 4297
页数：3
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