DNA fragmentation and morphological changes in apoptotic human lymphocytes

被引：45

作者：

Czene, S ^{[1
]}

Testa, E

Nygren, J

Belyaev, I

Harms-Ringdahl, M

机构：

[1] Univ Stockholm, Dept Genet & Cellular Toxicol, S-10691 Stockholm, Sweden

[2] Slovak Acad Sci, Canc Res Inst, Bratislava 83391, Slovakia

[3] Univ Tuscia, Dipartimento Agrobiol & Agrochim, I-01100 Viterbo, Italy

[4] Sodertorns Hogskola, SE-14104 Huddinge, Sweden

[5] Karolinska Inst, Dept Biosci Novum, SE-14157 Huddinge, Sweden

[6] Moscow Engn Phys Inst, Dept Radiat Phys Biophys & Ecol, Moscow 115409, Russia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 294卷 / 04期

关键词：

lymphocyte; radiation; apoptosis; DNA fragmentation; cell shrinkage; comet assay; pulsed field gel electrophoresis;

D O I：

10.1016/S0006-291X(02)00588-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell suspensions enriched in cells at various stages of apoptosis were obtained by separation of irradiated human peripheral blood lymphocytes on density gradients at different post-irradiation times. The state of DNA fragmentation in the cells was determined by comet assay and pulsed field gel electrophoresis. The morphologically distinguishable features of apoptosis such as chromatin condensation and cell shrinkage correlated with discrete stages of DNA fragmentation. It was found that greater than or equal to50kbp fragmentation of DNA occurs already in cells of normal density whereas the subsequent DNA fragmentation onto fragments <50 kbp occurs in parallel with cell shrinkage and simultaneous increase in cell density. The observed stages of DNA fragmentation seem to be separated in time that could allow in case of abortive apoptosis formation of chromosomal aberrations. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：872 / 878

页数：7

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