Influence of the spray adjuvant on the toxicity effects of a glyphosate formulation

被引:43
作者
Coalova, Isis [1 ]
Rios de Molina, Maria del Carmen [1 ]
Chaufan, Gabriela [1 ]
机构
[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Biol, IQUIBICEN CONICET, RA-1428 Buenos Aires, DF, Argentina
关键词
Mixture toxicity; Spray adjuvant; In vitro; Oxidative stress; OXIDATIVE STRESS; NONYLPHENOL; SURFACTANTS; GLUTATHIONE; CYTOTOXICITY; ALKYLPHENOLS; PESTICIDES; HERBICIDES; MIXTURE; HACAT;
D O I
10.1016/j.tiv.2014.06.014
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
In the present study, the influence of the spray adjuvant on the toxicity effects of a glyphosate formulation was examined in HEp-2 cell line. We determined the median lethal concentration (LC50) of Atanor (R) (glyphosate formulation), Impacto (R) (spray adjuvant) and the mixture of both agrochemicals. We also compared the toxicities of the pesticides individually and in mixture and we analyzed the effects on oxidative balance from each treatment. Our results showed that all the agrochemicals assayed induce dose and time-dependent cytotoxicity and that the toxicity of Impacto (R) with Atanor (R) (mixture) was additive on HEp-2 cell line. All the agrochemicals assayed produced an increase in catalase activity and glutathione levels, while no effects were observed for superoxide dismutase and glutathione-S-transferase activities. We found an important increase in ROS production in cells treated with Atanor (R) and mixture. Besides, all the agrochemicals used triggered caspase 3/7 activation and hence induced apoptosis pathway in this cell line. In conclusion, our results demonstrated that the addition of adjuvant to glyphosate formulation increase the toxicity of the mixture in cell culture. Furthermore, cell culture exposed to agrochemical mixture showed an increased ROS production and antioxidant defenses. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1306 / 1311
页数:6
相关论文
共 48 条
[1]   Glyphosate biomonitoring for farmers and their families: Results from the farm family exposure study [J].
Acquavella, JF ;
Alexander, BH ;
Mandel, JS ;
Gustin, C ;
Baker, B ;
Chapman, P ;
Bleeke, M .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2004, 112 (03) :321-326
[2]  
Aebi H, 1984, Methods Enzymol, V105, P121
[3]   Evaluation of genetic damage induced by glyphosate isopropylamine salt using Tradescantia bioassays [J].
Alvarez-Moya, Carlos ;
Reynoso Silva, Monica ;
Villalobos Arambula, Alma Rosa ;
Islas Sandoval, Alfonso ;
Castaneda Vasquez, Hugo ;
Gonzalez Montes, Rosa Maria .
GENETICS AND MOLECULAR BIOLOGY, 2011, 34 (01) :127-130
[4]  
ANDERSON ME, 1985, METHOD ENZYMOL, V113, P548
[5]   SUPEROXIDE DISMUTASE - IMPROVED ASSAYS AND AN ASSAY APPLICABLE TO ACRYLAMIDE GELS [J].
BEAUCHAM.C ;
FRIDOVIC.I .
ANALYTICAL BIOCHEMISTRY, 1971, 44 (01) :276-&
[6]   Toxicity of Formulated Glyphosate (Glyphos) and Cosmo-Flux to Larval and Juvenile Colombian Frogs 2. Field and Laboratory Microcosm Acute Toxicity [J].
Bernal, M. H. ;
Solomon, K. R. ;
Carrasquilla, G. .
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 2009, 72 (15-16) :966-973
[7]   Phthalates and nonylphenols in urban runoff: Occurrence, distribution and area emission factors [J].
Bjorklund, Karin ;
Cousins, Anna Palm ;
Stromvall, Ann-Margret ;
Malmqvist, Per-Arne .
SCIENCE OF THE TOTAL ENVIRONMENT, 2009, 407 (16) :4665-4672
[8]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[9]   Toxicity of hexachlorobenzene and its transference from microalgae (Chlorella kessleri) to crabs (Chasmagnathus granulatus) [J].
Chaufan, Gabriela ;
Juarez, Angela ;
Basack, Silvana ;
Ithuralde, Esteban ;
Sabatini, Sebastian E. ;
Genovese, Griselda ;
Oneto, Maria L. ;
Kesten, Eva ;
Rios de Molina, Maria del Camen .
TOXICOLOGY, 2006, 227 (03) :262-270
[10]   Glyphosate Commercial Formulation Causes Cytotoxicity, Oxidative Effects, and Apoptosis on Human Cells: Differences With its Active Ingredient [J].
Chaufan, Gabriela ;
Coalova, Isis ;
del Carmen Rios de Molina, Maria .
INTERNATIONAL JOURNAL OF TOXICOLOGY, 2014, 33 (01) :29-38