Rapid spectrofluorimetric method for traces of gibberellin

被引：7

作者：

Jiang, ZL

Liu, XH

Liang, AH

机构：

[1] Guangxi Normal University

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 346卷 / 02期

关键词：

spectrofluorimetry; gibberellin; perchloric acid;

D O I：

10.1016/S0003-2670(97)00025-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method for determination of gibberellin has been developed, based on the formation of a fluorescent compound with perchloric acid at 100 degrees C for 6 min. The maximum excitation and emission wavelengths of the fluorescent compound are 416 nm and 457 nm, respectively. Gibberellin in the range 10-800 ng ml(-1) can be determined; the 3 sigma detection limit is 5 ng ml(-1). The recommended method is very sensitive, selective, simple and rapid, making it ideal for the routine determination of gibberellin. The fluorescence reaction mechanism is discussed.

引用

页码：249 / 252

页数：4