B cell-dependent T cell responses: IgM antibodies are required to elicit contact sensitivity

被引:106
作者
Tsuji, RF
Szczepanik, M
Kawikova, I
Paliwal, V
Campos, RA
Itakura, A
Akahira-Azuma, M
Baumgarth, N
Herzenberg, LA
Askenase, PW
机构
[1] Yale Univ, Sch Med, Dept Internal Med, Allergy & Clin Immunol Sect, New Haven, CT 06520 USA
[2] Noda Inst Sci Res, Chiba 278, Japan
[3] Jagiellonian Univ, Coll Med, Dept Immunol, PL-31008 Krakow, Poland
[4] Univ Calif Davis, Ctr Comparat Med, Davis, CA 95616 USA
[5] Stanford Univ, Sch Med, Beckman Ctr, Dept Genet, Stanford, CA 94305 USA
关键词
T cell recruitment; complement C5 and C5a; skin immunity; IgM response; T and B cell interactions;
D O I
10.1084/jem.20020649
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Contact sensitivity (CS) is a classic example of in vivo T cell immunity in which skin sensitization with reactive hapten leads to immunized T cells, which are then recruited locally to mediate antigen-specific inflammation after subsequent skin challenge. We have previously shown that T cell recruitment in CS is triggered by local activation of complement, which generates C5a that triggers C5a receptors most likely on mast cells. Here, we show that B-1 cell-derived antihapten IgM antibodies generated within 1 day (d) of immunization combine with local challenge antigen to activate complement to recruit the T cells. These findings overturn three widely accepted immune response paradigms by showing that (a) specific Igm antibodies are required to initiate CS, which is a classical model of T cell immunity thought exclusively due to T cells, (b) CS priming induces production of specific IgM antibodies within 1 d, although primary antibody responses typically begin by day 4, and (c) B-1 cells produce the 1-d IgM response to CS priming, although these cells generally are thought to be nonresponsive to antigenic stimulation. Coupled with previous evidence, our findings indicate that the elicitation of CS is initiated by rapidly formed IgM antibodies. The Igm and challenge antigen likely form local complexes that activate complement, generating C5a, leading to local vascular activation to recruit the antigen-primed effector T cells that mediate the CS response.
引用
收藏
页码:1277 / 1290
页数:14
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