Development and characterization of polymorphic microsatellite markers in Linum usitatissimum

被引：35

作者：

Deng, Xin ^{[1
]}

Long, Songhua ^{[1
]}

He, Dongfeng ^{[2
]}

Li, Xiang ^{[2
]}

Wang, Yufu ^{[1
]}

Liu, Jia ^{[2
]}

Chen, Xinbo ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China

[2] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China

来源：

JOURNAL OF PLANT RESEARCH | 2010年 / 123卷 / 01期

关键词：

Linum usitatissimum; Microsatellites marker; Polymorphism;

D O I：

10.1007/s10265-009-0271-3

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, and observed and expected heterozygosities ranging from 0.125 to 0.375 (mean 0.013) and from 0.233 to 0.842 (mean 0.601), respectively. These polymorphic new microsatellite loci will be useful for genetic linkage map construction, germplasm classification and identification, gene identification and quantitative trait loci mapping, and marker-assisted selection in breeding in L. usitatissimum.

引用

页码：119 / 123

页数：5

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