Protective effect of green tea extract and tea polyphenols against the cytotoxicity of 1,4-naphthoquinone in isolated rat hepatocytes

被引:28
作者
Miyagawa, C
Wu, C
Kennedy, DO
Nakatani, T
Ohtani, K
Sakanaka, S
Kim, M
MatsuiYuasa, I
机构
[1] OSAKA CITY UNIV, FAC HUMAN LIFE SCI, DEPT FOOD & NUTR, SUMIYOSHI KU, OSAKA 558, JAPAN
[2] TAIYO KAGAKU CO LTD, CENT RES LAB, YOKKAICHI, MIE 510, JAPAN
关键词
green tea extract; tea polyphenols; 1,4-naphthoquinone; protein-SH; hepatocytes;
D O I
10.1271/bbb.61.1901
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cytoprotective effect of green tea extract and its phenolic compounds against 1,4-naphthoquinone-induced hepatotoxicity was evaluated in primary cultured rat hepatocytes. After exposure to 1,4-naphthoquinone, lactate dehydrogenase (LDH) leakage and cell viability were both improved by the presence of the tea extract and tea polyphenols. This cytoprotective effect was related to the structure of tea polyphenols, the galloyl group of (-)-epigallocatechin-3-gallate and (-)-epicatechin-3-gallate being particularly effective. The production of liquid peroxidation by 1,4-naphthoquinone was not inhibited by the tea extract nor by tea polyphenol addition. After 2 h of incubation, the protein thiol concentration was reduced by 1,4-naphthoquinone, but this reduction was prevented by the tea extract and tea polyphenols. The reduction in protein thiol content of the cells closely paralleled the LDH leakage and loss of cell viability. These results suggest that the mechanism of protection by tea polyphenols against 1,4-naphthoquinone-induced toxicity to rat hepatocytes was due to the maintenance of protein thiol levels.
引用
收藏
页码:1901 / 1905
页数:5
相关论文
共 22 条
[1]  
AGARWAL R, 1992, CANCER RES, V52, P3582
[2]  
ANON MT, 1992, Z NATURFORSCH C, V47, P275
[3]  
BERGMEYER H.U., 1974, METHODS ENZYMATIC AN, P574, DOI DOI 10.1016/B978-0-12-091302-2.50010-4
[4]  
Buege J A, 1978, Methods Enzymol, V52, P302
[5]   ALTERATIONS IN INTRACELLULAR THIOL HOMEOSTASIS DURING THE METABOLISM OF MENADIONE BY ISOLATED RAT HEPATOCYTES [J].
DIMONTE, D ;
ROSS, D ;
BELLOMO, G ;
EKLOW, L ;
ORRENIUS, S .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1984, 235 (02) :334-342
[6]   MENADIONE-INDUCED CYTO-TOXICITY IS ASSOCIATED WITH PROTEIN THIOL OXIDATION AND ALTERATION IN INTRACELLULAR CA-2+ HOMEOSTASIS [J].
DIMONTE, D ;
BELLOMO, G ;
THOR, H ;
NICOTERA, P ;
ORRENIUS, S .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1984, 235 (02) :343-350
[7]   GREEN TEA COMPOSITION, CONSUMPTION, AND POLYPHENOL CHEMISTRY [J].
GRAHAM, HN .
PREVENTIVE MEDICINE, 1992, 21 (03) :334-350
[8]   REDUCTION OF CHRONIC PSYCHOSOCIAL HYPERTENSION IN MICE BY DECAFFEINATED TEA [J].
HENRY, JP ;
STEPHENSLARSON, P .
HYPERTENSION, 1984, 6 (03) :437-444
[9]  
HIKINO H, 1985, J ETHNOPHARMACOL, V14, P19
[10]   CROSS-SECTIONAL STUDY OF EFFECTS OF DRINKING GREEN TEA ON CARDIOVASCULAR AND LIVER-DISEASES [J].
IMAI, K ;
NAKACHI, K .
BRITISH MEDICAL JOURNAL, 1995, 310 (6981) :693-696