Detection and quantitation of genetically modified maize (Bt-176 Transgenic maize) by applying ligation detection reaction and universal array technology

被引：24

作者：

Bordoni, R

Mezzelani, A

Consolandi, C

Frosini, A

Rizzi, E

Castiglioni, B

Salati, C

Marmiroli, N

Marchelli, R

Bernardi, LR

Battaglia, C

De Bellis, G

机构：

[1] Univ Milan, LITA, Consiglio Nazl Ric, Ist Tecnol Biomed, I-20090 Segrate, MI, Italy

[2] Univ Milan, LITA, Dipartimento Sci & Tecnol Biomed, I-20090 Segrate, MI, Italy

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2004年 / 52卷 / 05期

关键词：

genetically modified organisms; GMOs; polymerase chain reaction; PCR; ligation detection reaction; LDR; universal array; Bt-176 transgenic maize;

D O I：

10.1021/jf034871e

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

We have applied the ligation detection reaction (LDR) combined with a universal array approach to the detection and quantitation of the polymerase chain reaction (PCR) amplified cry1A(b) gene from Bt-176 transgenic maize. We demonstrated excellent specificity and high sensitivity. Down to 0.5 fmol (nearly 60 pg) of PCR amplified transgenic material was inequivocally detected with excellent linearity within the 0.1-2.0% range with respect to wild-type maize. We suggest the feasibility of extending the LDR/universal array format to detect in parallel several transgenic sequences that are being developed for food applications.

引用

页码：1049 / 1054

页数：6

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