arsRBOCT arsenic resistance system encoded by linear plasmid pHZ227 in Streptomyces sp strain FR-008

被引：53

作者：

Wang, Lianrong

Chen, Shi

Xiao, Xiang

Huang, Xi

Zhou, Delin

Zhou, Xiufen

Deng, Zixin

机构：

[1] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200030, Peoples R China

[2] Shanghai Jiao Tong Univ, Lab Microbial Metab, Shanghai 200030, Peoples R China

[3] Huazhong Agr Univ, Sch Life Sci & Technol, Wuhan 430070, Peoples R China

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 05期

关键词：

D O I：

10.1128/AEM.72.5.3738-3742.2006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the arsenic resistance gene cluster from the large linear plasmid pHZ227, two novel genes, arsO (for a putative flavin-binding monooxygenase) and arsT (for a putative thioredoxin reductase), were coactivated and cotranscribed with arsR1-arsB and arsC, respectively. Deletion of the ars gene cluster on pHZ227 in Streptomyces sp. strain FR-008 resulted in sensitivity to arsenic, and heterologous expression of the ars gene cluster in the arsenic-sensitive Streptomyces strains conferred resistance on the new hosts. The pHZ227 ArsB protein showed homology to the yeast arsenite transporter Acr3p. The pHZ227 ArsC appears to be a bacterial thioredoxin-dependent ArsC-type arsenate reductase with four conserved cysteine thioredoxin-requiring motifs.

引用

页码：3738 / 3742

页数：5

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