Phorbol ester-sensitive phospholipase D is mainly localized in the endoplasmic reticulum of BHK cells

被引：16

作者：

Decker, C ^{[1
]}

Obradors, MJM ^{[1
]}

Sillence, DJ ^{[1
]}

Allan, D ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL,DEPT PHYSIOL,LONDON WC1E 6JJ,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1996年 / 320卷

关键词：

D O I：

10.1042/bj3200885

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The localization of phorbol ester-sensitive phospholipase D (PLD) in baby hamster kidney cells has been investigated by determining the subcellular distribution of the phosphatidylbutanol produced when the cells are incubated with phorbol 12-myristate 13-acetate and n-butanol. Results derived by isolation of plasma membrane vesicles from intact cells or by subcellular fractionation on a sucrose density gradient suggest the PLD is specific for phosphatidylcholine and its primary site of action is not the plasma membrane but the endoplasmic reticulum.

引用

页码：885 / 890

页数：6

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